响应面法优化黑木耳菌液体发酵胞外多糖条件  

Response Surface Method for Optimizing the Conditions of Extracellular Polysaccharide in Liquid Fermentation of Black Fungus

作　　者：李洋[1] 宗希明[1] 袁寰[1] 史伟国[1] 李野[1] 王丽红[1] 杜娟[1] 周彤[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154002

出　　处：《安徽农业科学》2015年第33期159-160,187,共3页Journal of Anhui Agricultural Sciences

基　　金：林业公益性行业科研专项子项目(2010040401-02);黑龙江省教育厅科学技术研究项目(12511577);佳木斯大学科学技术研究重点项目(Sz2011-013);佳木斯大学研究生科技创新项目(LM2014-064);佳木斯大学大学生科技创新项目(XSYD2014-022)

摘　　要：[目的]优化液体发酵黑木耳菌胞外多糖的工艺条件。[方法]以黑木耳菌为试验材料,在单因素试验的基础上,采用苯酚-硫酸法对黑木耳菌液体发酵的胞外多糖含量进行测定,并采用响应面法优化黑木耳菌液体发酵胞外多糖的条件。[结果]试验表明,黑木耳菌液体发酵胞外多糖的最优条件为碳源可溶性淀粉3%、氮源胰蛋白胨0.15%、水果皮莎白瓜皮4%、中药材红景天10%,在此条件下的黑木耳菌液体发酵胞外多糖含量为0.024 51 g/ml。[结论]此工艺合理可行,可为开发利用黑木耳多糖提供基础数据和理论依据。[Objective] To optimize conditions of liquid fermentation of black fungus extracellular polysaccharide.[Method]Based on the single factor experiment,the contents of extracellular polysaccharide in the liquid fermentation of Aspergillus fungus were determined by the method of phenol sulfuric acid.[Result] The results showed that black fungus liquid fermentation of extracellular polysaccharide of optimal conditions for carbon source 3% soluble starch,nitrogen pancreatic peptone 0. 15%,fruit Pisha pumpkin skin 4%,Chinese herbal medicine Rhodiola 10%,under this condition,the content of extracellular polysaccharide was 0. 024 51 g / ml. [Conclusion] This process is reasonable and feasible,which provides the basic data and theoretical basis for the development and utilization of black fungus polysaccharide.

关 键 词：黑木耳 多糖 液体发酵 响应面法 

分 类 号：S38[农业科学—农艺学]