硝酸铈对鲤鱼鳃及消化道黏液细胞的影响  

Effect of Cerium Nitrate on Mucous Cells of Gills and Digestive Tract in Cyprinus carpio Linnaeus

作　　者：张贵生[1] 吴红松[1] 楚德昌[1] 

机构地区：[1]菏泽学院生命科学系,山东菏泽274015

出　　处：《安徽农业科学》2015年第34期217-218,243,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省教育厅科研发展计划项目(J08LF54);山东省生理生化与应用重点实验室项目

摘　　要：[目的]为稀土元素在渔业养殖上的科学应用提供科学依据。[方法]在饲料中添加20、42、65 mg/kg的硝酸铈饲喂鲤鱼30 d,利用AB-PAS染色方法研究铈对鲤鱼鳃及消化道各部位黏液细胞的影响。[结果]与对照组相比,各试验组鲤鱼鳃及口腔顶壁、前肠、中肠、后肠黏液细胞的密度提高。与对照组相比,各试验组鲤鱼鳃及消化道各部位Ⅱ型黏液细胞密度增加,Ⅳ型黏液细胞密度降低。此外,铈也能增加鳃丝、中肠Ⅲ型黏液细胞密度,降低鳃、前肠、中肠Ⅰ型黏液细胞密度。[结论]饲料中添加一定剂量的硝酸铈能显著增加鲤鱼鳃及消化道不同部位黏液细胞的密度。[Objective]The research aimed to provide basic basis for the scientific application of rare earth elements in the fishery farming.[Method]20,42 and 65 mg/kg cerium nitrate was added in the feed to feed Cyprinus carpio Linnaeus for 30 d. The effects of cerium nitrate on mucous cells of gills and digestive tract were studied by method of AB-PAS staining. [Result]Combined with control group,the densities of mucous cells in gill,top wall of oral cavity,foregut,midgut and hindgut of C. carpio in test groups were enhanced. Combined with control group,the densities of type Ⅱ mucous cells in the gill and foregut of C. carpio in test groups were increased,and the densities of type Ⅳmucous cells were decreased. In addition,the densities of type Ⅲ mucous cell in the gill and midgut of C. carpio in test groups were enhanced,and the densities of typeⅠmucous cell in the gill,foregut and midgut were decreased. [Conclusion]Adding some doses of cerium nitrate in the feed could significantly increase the densities of digestive tract of C. carpio.

关 键 词：硝酸铈 鲤鱼 鳃 消化道 黏液细胞 

分 类 号：S965.116[农业科学—水产养殖]