脂肪组织血管基质成分在组织工程化脂肪构建中的应用  

Application of stromal vascular fraction in engineered adipose tissue construction

作　　者：黄皎[1] 于湄[1] 田卫东[1] 

机构地区：[1]口腔疾病研究国家重点实验室华西口腔医院(四川大学),成都610041

出　　处：《国际口腔医学杂志》2016年第1期74-78,共5页International Journal of Stomatology

基　　金：国家自然科学基金(30973348);国家自然科学基金青年基金(81300848);四川省应用基础计划基金(2012JY0077)~~

摘　　要：脂肪组织血管基质成分(SVF)中含有脂肪干细胞并可提供一定的生长因子,在脂肪组织工程化构建领域相对于单独应用脂肪干细胞更具明显优势,近年来得到了广泛的应用。本文就SVF的组成和优势,SVF的具体提取方法和影响因素,SVF用于构建组织工程化脂肪中支架材料的选择,SVF包含的构建组织工程化脂肪的生长因子和将SVF用于构建组织工程化脂肪时应考虑到局部微环境的作用,SVF复合脂肪组织构建组织工程化脂肪,SVF复合富血小板血浆或富血小板血纤蛋白构建组织工程化脂肪的方法,以SVF构建组织工程化脂肪存在的问题等作一综述,旨在帮助临床构建出可用于治疗的存活率高并发症少的组织工程化脂肪,以推动整形外科的发展。The stromal vascular fraction contains adipose-derived stem cells and provides cytokines; this vascular fraction has been applied wildly in adipose tissue engineering field as a superior material compared with adipose-derived stem cells alone. This review illustrates the components and merits of stromal vascular fraction; summarizes stromal vascular fraction isolation methods and operation details; discusses the choices of scaffold materials; introduces cytokines in the micro-environment surrounding the stromal vascular fraction, which should be considered in engineered adipose tissue construction; analyzes two specific methods（i.e., mixture of the stromal vascular fraction and fat granules and mixture of the stromal vascular fraction and platelet-rich plasma or fibrin） to regenerate adipose tissue; and concludes with the obstacles of stromal vascular fraction in engineered adipose tissue construction. This review aims to contribute to the construction of engineered adipose tissue that can be applied in the clinical setting with higher survival rates and fewer complications.

关 键 词：血管基质成分 组织工程 脂肪干细胞 脂肪移植 

分 类 号：R622.9[医药卫生—整形外科]