牛支原体氟喹诺酮类药物耐药靶位突变分析  

Quinolones Resistance Targeted Mutation Analysis of Mycoplasma bovis

作　　者：贾博岩[1] 赵立峰[1] 高铎[1,2] 孔令聪[1] 刘树明[1] 王春凤[1] 马红霞[1,3] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]辽宁省兽药饲料畜产品质量安全检测中心,辽宁110000 [3]吉林农业大学动物生产及产品质量安全教育部重点实验室,长春130118

出　　处：《中国兽药杂志》2015年第12期1-5,共5页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金项目(31272611);吉林省科技发展计划项目任务书(20150101109JC)

摘　　要：为探讨牛支原体氟喹诺酮类药物耐药靶位的突变情况,对分离自我国多个省份的牛支原体进行氟喹诺酮类药物耐药性检测和耐药株筛选,通过对临床分离敏感株、耐药株及体外诱导高度耐药株的氟喹诺酮类药物耐药决定区(QRDR)进行测序分析,发现分离菌株中有19%(6/32株)对氟喹诺酮类药物耐药,其QRDR中均存在gyr A(Ser83Phe)或par C(Ser80Ile)的氨基酸突变;但在体外诱导的高度耐药株中QRDR突变类型则以gyr A(Ser83Phe/Tyr或Glu87Lys)和par C(Ser80Ile或Asp84Asn/Tyr)的氨基酸发生突变为主,以上靶位突变在介导牛支原体对氟喹诺酮类药物耐药水平方面是否起着决定性作用,还有待进一步证明。In order to discuss the quinolones resistance targeted mutation analysis of Mycoplasma bovis. In this study,Mycoplasma bovis isolated from several provinces were tested on quinolone resistance and quinolone resistant strains were screened. The quinolone resistance determining region（ QRDR） of clinical isolates sensitive,resistant strains and resistant strains induced in vitro were sequenced. The results showed that 19% isolates strains（ 6 /32） were resistant to quinolones,and it was found that the resistant strains possess Ser83 Phe in gyr A or ser80 Ile In par C. But it found that the QRDR mutation types based on gyr A（ Ser83 Phe / Tyr or Glu87Lys） and par C（ Ser80 Ile or Asp84 Asn / Tyr） in laboratory- derived resistant Mycoplasma bovis. The above targeted mutation either whether paly an important role in mediating the quinolone resistance level of Mycoplasma bovis will remains to be further proved.

关 键 词：牛支原体 氟喹诺酮类药物 靶位突变 

分 类 号：S852.62[农业科学—基础兽医学]