微波辅助酶法提取姜黄中姜黄素的工艺研究  

Study on Microwave- assisted Enzyme Extraction Process for Curcumin from Curcuma longa

作　　者：宁娜[1,2] 韩建军[1,2] 胡宇莉[3] 邹宗尧 郁建生[1,2] 

机构地区：[1]铜仁职业技术学院药学院,贵州铜仁554300 [2]贵州省中兽药工程研究中心,贵州铜仁554300 [3]重庆市动物疫病预防控制中心,重庆401120 [4]西南大学药学院,重庆400715

出　　处：《中国兽药杂志》2015年第12期20-26,共7页Chinese Journal of Veterinary Drug

基　　金：贵州省农业类工程技术研究中心项目(黔科合农G字[2015]4001号)

摘　　要：为了研究微波辅助酶法提取姜黄中姜黄素的工艺,以姜黄素收率为考核指标,在单因素试验的基础上,采用响应面分析法对姜黄素提取工艺进行优化。优选姜黄素的最佳酶解辅助提取工艺为:酶用量9.8 mg/g,酶解时间75 min,酶解p H值4.7,酶解温度43℃。所建立的提取工艺合理、稳定、可行,为进一步工业化生产提供理论依据。Curcumin from Curcuma longa was extracted by microwave- assisted enzyme extraction process. Based on the single factor experiment,the optimal extraction technology was optimized by response surface methodology with curcumin yield as the detective marker. The optimum extraction condition were as follows： cellulase dosage9. 8 mg / g,enzymolysis time 75 min,p H 4. 7,enzymolysis temperature 43 ℃. The extraction process is reasonable and scientific,and it could be used in future large- scaled preparation.

关 键 词：姜黄 姜黄素 纤维素酶 微波法 提取工艺 

分 类 号：S853.73[农业科学—临床兽医学]