MMP-3、Fn和α-SMA在糖尿病模型晶状体上皮细胞中的表达及意义  

The significance and expressions of MMP-3, Fn and α-SMA in lens epithelium cells of the experimental diabetic cataract rats

作　　者：杨莹莹[1] 何湘珍[2] 陶利娟[1] 

机构地区：[1]湖南省儿童医院眼科,长沙410007 [2]南华大学附属二医院眼科

出　　处：《中国医师杂志》2015年第12期1827-1831,共5页Journal of Chinese Physician

摘　　要：目的观察糖尿病性白内障大鼠晶状体上皮细胞（LECs）的病理变化，探讨基质金属蛋白-3（MMP-3）、α-平滑肌肌动蛋白（α-SMA）、纤维连接蛋白（Fn）在糖尿病性白内障发生、发展中的作用。方法将105只SD大鼠，随机分为2组，正常对照组45只、糖尿病模型组60只。模型组大鼠腹腔注射链脲佐菌素（STZ）溶液（60mg／kg）；正常对照组腹腔内注射相应体积的0．1mmol／L柠檬酸缓冲溶液。每周通过裂隙灯观察并记录大鼠晶状体混浊的进展。在建模后4、8、12周时取大鼠晶状体，制成石蜡切片，用HE染色观察LECs的变化情况，采用免疫组化法检测LECs中MMP-3、α-SMA、Fn的表达，并进行相关比较及统计学分析。结果正常对照组中，大鼠晶状体始终保持透明状态；而糖尿病模型组在实验第4、8、12周末白内障的发生率分别为31．48％、77．78％、100％。在光镜下糖尿病白内障组的LECs出现细胞聚集的斑块与成纤维细胞样改变，并形成多层排列。正常对照组LECs中，MMP-3、α-SMA、Fn均不表达；而在糖尿病性白内障组LECs中MMP-3、α-SMA、Fn的表达明显增高，与对照组比较差异有统计学意义（P〈0．01）。随着病程的发展，糖尿病性白内障组LECs中MMP-3、α-SMA、Fn阳性表达率呈进行性增强，各时间段之间阳性表达率差异有统计学意义（P〈0．05）。在糖尿病性白内障组中，LECs中Fn与α-SMA和MMP-3之间的表达呈正相关（r=0．994，P〈0．01；r=0．993，P〈0．01）。结论α-SMA、MMP-3和Fn可能通过调节LECs的生长在糖尿病性白内障的发生、发展中起重要作用。Objective To investigate pathological changes of lens epithelium cells （LECs） and expressions of matrix metalloproteinase-3 （ MMP-3 ） , alpha-smooth muscle actin （α-SMA） , and fibroneetin （Fn） in lens epithelial cells of experimental diabetic cataract rats, and to evaluate the roles of MMP-3,α-SMA, and Fn in the pathogenesis of diabetic cataract. Methods A total of 105 healthy Sprague-Dawley （SD） rats without lens diseases was randomly divided into normal control （ n = 45 ） and diabetic model （ n = 60） groups. Diabetic model rats were subjected to intraperitoneal injection of streptozotocin （STZ） （60 mg/kg）, and those in the normal control group received injection with 0. 1 mmol/L citric acid buffer solution of the same volume. The diabetic models were affirmed upon a fasting blood ≥ 16. 65 mmol/L at the 3rd days after the injection. Once a week, the changes of blood glucose and body weight were monitored and the progression of cataract formation in both lenses of all rats was recorded with slit lamp observation. At end of 4 weeks, 8 weeks, and 12 weeks after STZ injection, lenses were isolated and embedded in paraffin. The LECs histopathology was examined with HE staining. Immunohistochemical method was used to detect expressions of MMP-3, tx-SMA, and Fn in normal and diabetic LECs. The related comparisons and statistic α-nalysis were carried out. Results The lenses of control group were always completely transparent throughout the period of experiment with 31.48% , 77. 78% , and 100% of lenses in diabetic model group swelling at 4th, 8th, and 12th week, respectively. Under the light microscopic level, it has been showed that lens epithelium cells, which was occurred aggregate plaque and arranged in many layers, presented some morphologic characteristics of fibroblasts by HE staining. In control group LECs regularly, MMP-3, α-SMA, and Fn did not express equally; MMP-3, α-SMA, and Fn expressions increased obviously highly, difference hact statistical significance compare

关 键 词：白内障/并发症/代谢 糖尿病并发症/代谢 晶体/细胞学/病理学 上皮细胞/病理学 基质金属蛋白酶3/代谢 肌动蛋白类/代谢 纤连蛋白类/代谢 

分 类 号：R587.2[医药卫生—内分泌] R776.1[医药卫生—内科学] R-332[医药卫生—临床医学]