灰树花提取物抗肝癌活性及初步机制研究  

Anti-Hepatocellular Carcinoma Effects of Grifola frondosa Extract and Its Underlying Mechanisms

作　　者：胡馨予[1] 胡爽[2] 张峻榕 殷梦雅 周毓麟[2] 

机构地区：[1]长春医学高等专科学校临床医学部,长春130013 [2]吉林大学生命科学学院,长春130012

出　　处：《中国药学杂志》2015年第24期2107-2111,共5页Chinese Pharmaceutical Journal

基　　金：教育部科技支撑项目基金资助项目(2012BAL29B05)

摘　　要：目的考察灰树花提取物抗肝癌作用及相关分子机制。方法在PLC/PRF/5和HepG2细胞中,采用灰树花提取物处理给药法,噻唑蓝法测定细胞存活率,化学法测定乳酸脱氢酶释放及Capase3活力,JC-1染色测定线粒体跨膜电位变化,Western Blot测定Bcl-2和Bax蛋白表达以及Akt/GSK3β磷酸化情况。在PLC/PRF/5异位荷瘤裸鼠模型中,灰树花的抗肝癌效果得到进一步验证。结果灰树花提取物可以有效降低PLC/PRF/5和HepG2细胞存活率,提高细胞乳酸脱氢酶释放,增加Caspase3活力,减弱线粒体跨膜电位,增加Bax表达,降低Bcl-2蛋白含量,降低Akt/GSK3β磷酸化水平。在不改变体重的情况下,灰树花可有效抑制在PLC/PRF/5异位荷瘤裸鼠模型中肿瘤的生长。结论灰树花可通过线粒体依赖途径及Akt/GSK3β通路有效起到抗肝癌活性。OBJECTIVE To investigate the anti-hepatocellular carcinoma effect and underlying mechanisms. METHODS In PLC / PRF /5 and HepG2,after treatment with Grifola frondosa extract,MTT method,chemical method,JC-1 staining and Western Blot were applied to determine cell viability,caspase 3 activity,mitochondrial membrane potential,the expression of Bcl-2 and Bax,and the phosphorylation of Akt / GSK3β. The anti-tumor activity of Grifola frondosa extract was further confirmed in PLC / PRL /5-xengrafted mice model. RESULTS Grifola frondosa extract significantly reduced cell viability,mitochondrial membrane potential,the expression of Bcl-2 and the phosphorylation of Akt / GSK3β,and enhanced LDH release,caspase 3 activity and the expression of Bax in both PLC / PRF /5 and Hep G2 cells. 12-day Grifola frondosa extract treatment significantly inhibited the PLC / PRF /5-xenografted tumor growth without influence the body weight of mouse. CONCLUSION All these data indicate that Grifola frondosa extractmediated anti-hepatocellular carcinoma effects are related to its modulation of the activations of Akt / GSK3 β and mitochondrial pathway.

关 键 词：灰树花 抗肝癌 线粒体 AKT/GSK-3beta 

分 类 号：R965[医药卫生—药理学]