不同相对分子质量海带岩藻聚糖硫酸酯内皮细胞保护作用比较及机制探讨  

A Comparative Study of Protective Action on Endotheliocytes of Fucoidan with Different Molecular Weight and Its Mechanism

作　　者：陈安进[1] 张芳[1] 石杰[1] 闫美兴[1] 

机构地区：[1]青岛市市立医院药学部,山东青岛266071

出　　处：《中国药学杂志》2015年第24期2112-2116,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30800858);山东省自然科学基金资助项目(ZR2010CQ020)

摘　　要：目的考察不同相对分子质量岩藻聚糖硫酸酯对内皮细胞的保护作用并探讨其机制。方法采用体内研究和体外研究的方法,探讨不同相对分子质量海带岩藻聚糖硫酸酯对内皮细胞的保护作用。在体内研究中,通过制作大鼠内皮损伤模型,显微镜下观察大鼠胸主动脉内皮损伤情况,并测定内皮损伤指标血管性血友病因子(vWF)。在体外研究中,通过细胞培养,以血管性血友病因子和内皮微颗粒(EMPs)水平作为内皮损伤指标,考察药物对内皮损伤的保护作用。结果体内研究:光学显微镜下HE切片观察显示,相对低相对分子质量组(LF1组和LF2组)对内皮细胞的保护作用强于相对中相对分子质量组(MFa组和MFb组)。与模型组比较,LF1和LF2可显著降低血管性血友病因子水平(P<0.05),而MFa和MFb与模型组无显著性差异(P>0.05)。体外研究:与模型组比较,LF1和LF2可显著降低血管性血友病因子水平(P<0.05),而MFa和MFb与模型组无显著性差异(P>0.05)。LF1和LF2组的内皮微颗粒数目显著低于模型组(P<0.01),而与正常对照组无统计学差异;MFa和MFb组的内皮微颗粒数目比正常对照组明显增多(P<0.01),数目虽比模型组有所减少,但无统计学差异(P>0.05)。结论低相对分子质量的岩藻聚糖硫酸酯对内皮细胞均具有良好的保护作用,其通过降低血管性血友病因子和内皮微颗粒浓度,阻断血小板与受损血管内皮中的胶原结合,从而抑制了血小板活化,发挥内皮保护作用;而相对中相对分子质量的岩藻聚糖硫酸酯对内皮细胞的损伤改善不明显。OBJECTIVE To study the protective action on endotheliocytes of fucoidan with different molecular weight from Laminaria japonica,and to explore its mechanism. METHODS In vivo,endotheliocytes injury model in rats was used to examine the protective action on endotheliocytes of fucoidan with different molecular weight from Laminaria japonica; aortal endothelium section by HE stain under optical microscope was observed,and the the content of von willebrand factor（ vWF） in plasma was determined by ELISA.In vitro,endothelial cell culture was used to determine the effect of fucoidan on content of v WF and endothelial microparticles（ EMPs）. RESULTS In vivo,aortal endothelium section by HE stain under optical microscope showed low molecular weight（ LMW） fucoidan had a remarkable protective effect,and the protection effect of LMWF fucoidans are stronger than middle molecular weight（ MMW） fucoidans. Administration of LMW fucoidan significantly decreased the v WF content. Administration of MMW fucoidan didn＇t change v WF content. In vitro,Administration of LMW fucoidan significantly decreased the v WF content and EMPs numbers. Administration of MMW fucoidan didn＇t change the vWF content and EMPs numbers. CONCLUSION The LMW fucoidan fractions have good protective action on endothelial cells. The protective effect of LMW fucoidans is significantly stronger than MMW fucoidans. The protective action on endotheliocytes of LMW fucoidans relies on decreasing the concentration of EMPs and v WF,and thus inhibiting platelet activation indirectly.

关 键 词：岩藻聚糖硫酸酯 内皮细胞 血管性血友病银子 内皮微颗粒 肾上腺素 

分 类 号：R965[医药卫生—药理学]