深圳H7N9流感病毒PB1-F2基因分子特征  

Molecular characteristics of the PB1-F2 gene in H7N9 influenza virus in Shenzhen

作　　者：董方圆[1] 王昕[2] 彭博[2] 武伟华[2] 刘慧[2] 丁小满 郑青[1] 房师松[2] 

机构地区：[1]暨南大学药学院,广州510632 [2]深圳市疾病预防控制中心

出　　处：《中华实验和临床病毒学杂志》2015年第6期493-497,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：深圳市科技计划项目（JCYJ20140410164811662）

摘　　要：目的了解深圳20株H7N9流感病毒PB1-F2基因的分子特征。方法使用DNAstar、MEGA等生物软件对深圳地区H7N9流感病毒PB1-F2进行核苷酸、氨基酸序列同源性和系统进化分析。结果2014年底到2015年H7N9流感病毒爆发主要集中在广东省，以深圳居首。深圳20株H7N9流感病毒PB1-F2基因主要分为三个亚系。3株的PB1-F2基因的N端缺失编码52个氨基酸；1株PB1-F2基因的c端缺失编码57个氨基酸；1株PB1-F2基因的c端缺编码76个氨基酸；其余株PB1-F2基因编码90个氮基酸。各深圳株与A／Anhui／1-DEWH730／2013株相比核苷酸和氨基酸的同源性分别为96．0％-100％和89．O％～100％。各深圳株之间的核苷酸同源性和氨基酸同源性分别为94．9％～100％和85．7％～100％。结论深圳H7N9流感病毒PB1-F2基因具有独特的分子特征。Objective To find out the virus isolated in Shenzhen district. Methods molecular characteristics of PB1-F2 DNAstar7.1.0 and MEGA6. 06 gene in H7N9 influenza were used to conduct sequence alignments and phylogentic analysis. Results From the end of 2014 to 2015, H7N9 virus infections have been reported mainly in Guangdong province, among which H7N9 cases in Shenzhen were the most. 20 sequences of PB1-F2 gene of H7N9 viruses isolated in Shenzhen were divided into three lineages. Of these sequences, 3 sequences encode an N-terminally truncated PB1-F2 （52aa）polypeptide, 1 sequence encode C-terminally truncated PB1-F2 （57aa）polypeptide, 1 sequence encode C-terminally truncated PB1- F2 （76aa）polypeptide respectively. The remaining sequences encode a full-length PBI-F2 （90aa）. The pairwise identities between the Shenzhen H7N9 strains and A/Anhui/1/2013 were 96.0% - 100% for nucleotides and 89.0% - 100% for the translated amino acids. The pairwise identities among the Shenzhen H7N9 strains were 94. 9%-100% for nucleotides and 85.7%-100% for the translated amino acids. Conclusion H7N9 influenza virus isolated in Shenzhen district has its unique molecular characteristics.

关 键 词：甲型流感病毒H7N9 PB1-F2基因 进化分析 

分 类 号：R373.13[医药卫生—病原生物学]