一种快检试剂对不同基因型诺如病毒的检测评价  

Evaluation of a rapid test kit for noroviruses of different genotypes

作　　者：高志勇[1] 严寒秋[1] 刘白薇[1] 霍达[1] 杜轶威 王全意[1] 

机构地区：[1]北京市疾病预防控制中心传染病地方病控制所食物中毒诊断溯源技术北京市重点实验室,100013

出　　处：《中华实验和临床病毒学杂志》2015年第6期547-550,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：北京市自然科学基金项目（7132045）

摘　　要：目的评价一种快检试剂对不同基因型诺如病毒的检测效果。方法收集2011年4月到2015年5月诺如病毒核酸阳性的腹泻患者粪便标本101份，用荧光定量RT—PCR检测GI／GII组诺如病毒核酸。用逆转录聚合酶链反应（RT-PCR）扩增衣壳蛋白区基因，测序、进化分析确定基因型。以荧光定量RT．PCR检测和基因型分型结果为参照，对德国拜发公司免疫层析法快速检测试剂（RIDA QUICKNorovirus）进行评价。结果101份标本中，荧光定量RT—PCR检测出8份GI，93份GⅡ。RIDA QUICK检出1份GI，57份GII，符合率57．4％。RIDA QUICK可检出GI．9、GII．3、GII．4、GⅡ．6、GⅡ7、GII．13、GII．17、GII．21和GⅡN，不能检出GI．2、GI．6、GI．N和GⅡ．2。RIDA QUICK对GII．4型诺如病毒的检出率较高（77．8％，28／36），但对新的GII．17变异株检出率较低（38．1％，8／21）。RIDA QUICK阳性的条带深浅度与荧光定量RT—PCR平均Ct值存在相关性（rs=-0．498）。结论RIDAQUICK能检出G11组诺如病毒的常见基因型，但对新出现的GⅡ-17变异株检出效果较差。Objective To evaluate the efficiency of a rapid immunoehromatographic test kit for noroviruses of different genotypes in fecal specimens. Methods Between April 2011 and May 2015, 101 norovirus-positive fecal specimens were collected from patients with diarrhea. Noroviruses of genogroup I （ G I ） or Ⅱ （ GⅡ ） were detected using the real time RT-PCR. The capsid genes of noroviruses were amplified using the RT-PCR, and genotypes were identified through the phylogenetic analysis. The results of real-time RT-PCR and genotyping were employed as the reference, then the rapid immunochromatography test kit （RIDA~ QUICK Norovirus） was evaluated. Results Among 101 specimens, 8 strains of GI and 93 strains of G ]I were identified by real time RT-PCR, whereas 1 strains of GI and 57 strains of GⅡ were identified by RIDAⅡ QUICK Norovirus, with the coincidence rate of 57.4%. RIDAⅡ QUICK Norovirus could detect GI. 9, GⅡ.3, GⅡ.4, GⅡ.6, GII7, GⅡ.13, GⅡ.17, GⅡ.21 and GⅡ.N, and it failed to detect GI. 2, GI. 6, GI. N and G Ⅱ~ 2. The detection rates for GⅡ . 4 strains and new G Ⅱ . 17 variants were 77.8% （28/36） and 38. 1% （8/21） respectively. Conclusion This assay was effective for common GII noroviruses, but less reliable for new G Ⅱ . 17 variants.

关 键 词：诺如病毒 免疫层析法 快速检测 基因型 

分 类 号：R440[医药卫生—诊断学]