免显微物镜的共光路相位显微成像方法  

Phase Microscopy Imaging Method Based on Common-Path without Micro-Objective

作　　者：陈映洲[1] 季颖[1] 谢铭[1] 徐媛媛[2] 韩豪[1] 卜敏[1] 王亚伟[1] 

机构地区：[1]江苏大学理学院,江苏镇江212013 [2]江苏大学机械工程学院,江苏镇江212013

出　　处：《激光与光电子学进展》2015年第12期154-159,共6页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(11374130;11474134;11404145);江苏省自然科学基金(BK20141296);中国博士后科学基金(2014M561574);江苏省普通高校研究生科研创新计划项目(KYLX_1017);江苏省博士后科研资助计划项目(1301148C);江苏省光子制造科学与技术重点实验室(江苏大学)开放基金课题(GZ201308)

摘　　要：相位显微成像技术在生物细胞形态观察和类别识别中有着重要的应用。基于稳定性强的共光路系统,提出了一种利用反射镜将光束一分为二,然后重叠,产生共光路干涉的方法,该方法利用光束的几何放大作用,免加载显微物镜,即可将干涉图像放大在成像屏上,且通过调节反射镜的角度,实现同轴、离轴和微轻离轴相位显微成像,对图像进行相位恢复处理后,可了解相位样品的形态结构,理论和实验结果表明该方法可行,为生物细胞相位显微成像新技术的开发研究提供了一种方法。Phase microscopic imaging technology has an important application significance in morphological identification as well as classification of biological cells. An interferometric imaging method is introduced, which is based on a high stability common-path interference system. In this approach, a reflecting mirror is employed to divide one beam into two parts. Then interferogram can be obtained by the overlap of these two beams. It is worth pointing out that, the interferogram can be amplified by means of geometrical magnification of the light instead of microobjective. Furthermore, the on-axis, off-axis and micro off-axis phase microscopy images can be obtained by controlling the angular position of the reflecting mirror. Then the structure of the sample can be acquired by phase retrieval. The method is suggested to be feasible by theoretical analysis and experiment result, which can provide a approach to phase microscopy imaging.

关 键 词：显微 相位成像 生物细胞 共光路 

分 类 号：O436.1[机械工程—光学工程]