白菜型油菜PDAT1基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of PDAT1 in Brassica rapa

作　　者：穆姣 赵翠珠[1] 王莉莉[1] 李卓蔚[1] 李欢[2] 刘明喆[1] 李长圣 杨铮[1] 张猛[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《西北农业学报》2015年第12期78-84,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：13115重大科技项目(2011KTZB02-01-03)~~

摘　　要：磷脂二酰甘油酰基转移酶(phospholipid:diacylglycerol acyltransferase 1,PDAT1)催化三酰甘油合成的最后一步反应,在生物质能源领域有良好的应用前景。本试验克隆得到白菜型油菜PDAT1的2条编码区序列,分别命名为BrPDAT1-1和BrPDAT1-2。BrPDAT1-1CDS全长2 007bp,编码668个氨基酸残基组成的肽链;BrPDAT1-2CDS全长1 794bp,编码597个氨基酸残基组成的肽链。生物信息学分析表明:BrPDAT1-1与AtPDAT1的相似度为91.08%,BrPDAT1-2与AtPDAT1的相似度为82.86%,两序列都编码一个无信号肽的亲水性稳定蛋白,跨膜结构域分析表明AtPDAT1和BrPDAT1-1都有1个跨膜结构域,而BrPDAT1-2无跨膜结构域。BrPDAT1-1和BrPDAT1-2蛋白序列均含有卵磷脂-胆固醇-酰基转移酶超家族保守结构域。Phospholipid. diacylglycerol acyltransferasel （PDAT1） catalyzes the final step of the synthesis of TAG ,and TAG has a good application prospect in field of biomass energy. In this study, two coding regions of PDAT1 of Brassica rapa were cloned, named BrPDATI-1 and BrPDAT1-2 respectively. The length of BrPDATI-1 was 2 007 bp, which encodeed a protein sequence composed of 668 amino acids. The length of BrPDAT1-2 was 1 794 bp, which encoded a protein sequence composed of 597 amino acids. Bioinformatics analysis indicated that similarity between BrPDATI-1 and AtPDAT1 was 91.08G, BrPDAT1-2 and AtPDAT1 was 82.86G. Both of the two sequences could encode stable proteins with no signal peptide, which had property of hydrophilicity. Transmembrane structure analysis showed that AtPDAT1 and BrPDATI-1 contained ltransmembrane domain respectively. However, BrPDAT1-2 contained no transmembrane domain. BrPDATI-1 and BrPDAT1-2 protein sequence contained phosphatidylcholine-sterol -acyltransferase superfamily conversed domain.

关 键 词：白菜型油菜 PDAT1 基因克隆 生物信息学 

分 类 号：S565.4[农业科学—作物学]