罗布麻查尔酮合成酶基因克隆及序列分析  

Cloning and Sequence Analysis of Chalcone Synthase Gene(CHS)from Apocynum venetum

作　　者：李苗[1,2] 李国旗[1] 

机构地区：[1]宁夏大学西北退化生态系统恢复与重建教育部重点实验室,银川750021 [2]宁夏农业生物技术重点实验室,银川750002

出　　处：《西北农业学报》2015年第12期85-91,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31160066);国家科技支撑项目(2011BAC07B03)~~

摘　　要：为了解罗布麻查尔酮合成酶基因具体结构，采用RT-PCR、RACE方法从夹竹桃科植物罗布麻中扩增出CHS基因的开放阅读框，其核苷酸序列长1170bp，推测的氨基酸序列全长为389个氨基酸残基。核苷酸序列同源性分析结果显示，该cDNA片段与其他植物CHS基因的同源性为78％～81％，表明该基因在进化过程中变异程度较小，整个超基因家族序列高度保守。The main purpose of this study was to determine the structure of CHS in Apocynum veneturn. Through RT-PCR（reverse transcription polymerase chain reaction） and RACE（rapid amplification of cDNA ends）, a fragment of 1 170 bp,coding 389 amino acids（aa）, was obtained from Apocynum venetum. The nucleotide sequence of AvCHS cDNA shared 78%-81% identity with that in the other family members, which suggested that the AvCHS genes has lesser variation during evolution, and highly conserved sequence existed in the supergene family.

关 键 词：罗布麻 CHS基因 基因克隆 

分 类 号：S563.7[农业科学—作物学]