慢性阻塞性肺疾病合并牙周炎患者血清辅助性T细胞及调节性T细胞表达对照分析  

Expression level analysis of peripheral regulatory T cells and helper T cells in chronic obstructive pulmonary disease patients with periodontitis

作　　者：李方治[1] 侯春阳[2] 王笑歌[3] 

机构地区：[1].中国医科大学附属第四医院呼吸内科,辽宁沈阳110032 [2]沈阳市第六人民医院内科,辽宁沈阳110006 [3]中国医科大学附属第四医院呼吸内科,辽宁沈阳110032

出　　处：《临床军医杂志》2015年第6期562-565,共4页Clinical Journal of Medical Officers

基　　金："十一五"国家科技支撑计划(2009BAI18B02)

摘　　要：目的对照分析慢性阻塞性肺疾病(COPD)合并牙周炎(PD)患者血清辅助性T细胞及调节性T细胞的表达现况。方法选择2013年1月至2014年6月于中国医科大学附属第四医院呼吸内科门诊就诊和住院治疗的COPD合并PD患者27例,同期COPD(不合并PD)患者24例,并选择同期于我院体检的健康人25例作为对照组。入选对象均在同一期间接受了血清辅助性T细胞(CD3+、CD4+、CD8+、CD4+/CD8+)及调节性T细胞(CD4+CD25+Treg和CD4+CD25+Foxp3+Treg)检测。结果 COPD合并PD患者血清CD3+、CD4+表达浓度、CD4+/CD8+比值以及CD4+CD25+Treg、CD4+CD25+Foxp3+Treg表达比例均显著低于COPD组及对照组,差异均有统计学意义(P<0.05)。COPD合并重度PD患者血清血清CD3+、CD4+表达浓度、CD4+/CD8+比值以及CD4+CD25+Treg、CD4+CD25+Foxp3+Treg表达比例均显著低于COPD合并中、轻度PD患者,差异均有统计学意义(P<0.05)。结论 COPD合并PD患者存在明确的血清调节性T细胞和辅助性T细胞低水平表达,且随着PD症状加重,上述趋势更加明显。Objective To investigate the expression levels of regulatory T cells（ CD4^＋CD25^＋Treg,CD4^＋CD25^＋Foxp3^＋Treg）and helper T cells（ CD3^＋,CD4^＋,CD8^＋,CD4^＋/ CD8^＋） of peripheral blood in chronic obstructive pulmonary disease（ COPD）patients with periodontitis（ PD）. Methods Totally 27 COPD patients with PD,24 COPD patients without PD and 25 healthy controls were selected from the outpatient and sickroom of respiratory department of the Fourth Affiliated Hospital of China Medical University during January 2013 to June 2014. CD4^＋CD25^＋Treg,CD4^＋CD25^＋Foxp3^＋Treg and CD3^＋,CD4^＋,CD8^＋,CD4^＋/CD8^＋in serum were detected in the 3 groups,respectively. Results The expression concentration of CD3^＋,CD4^＋,CD4^＋/CD8^＋ratio,CD4＋CD25＋Treg and CD4^＋CD25^＋Foxp3^＋Treg expression proportion of COPD patients with PD were significantly lower than those in the COPD patients without PD and control group（ P 〈0. 05）. The expression concentration of CD3^＋,CD4^＋,CD4^＋/ CD8^＋ratio,CD4＋CD25＋Treg and CD4^＋CD25^＋Foxp3^＋Treg expression proportion of COPD patients with severe PD were significantly lower than those with mild and moderate PD（ P 〈0. 05）. Conclusion Regulatory T cells and helper T cells of peripheral blood were low expressed in COPD patients with PD,and such abnormal expression became obvious with the aggravating symptom of PD.

关 键 词：慢性阻塞性肺疾病 牙周炎 调节性T细胞/血清 辅助性T细胞/血清 

分 类 号：R563.3[医药卫生—呼吸系统]