HCV-cAg和HCV-Ab联合检测对HCV感染诊断价值的研究  

Study on the diagnosis value of combination of HCV-cAg and anti-HCV in HCV infection

作　　者：林俊填[1] 余晋林[1] 伍伟健[1] 陈展泽[2] 龚道元[3] 

机构地区：[1]佛山市中心血站,广东佛山528000 [2]佛山市第一人民医院 [3]佛山科学技术学院医学院

出　　处：《实验与检验医学》2015年第6期721-723,共3页Experimental and Laboratory Medicine

基　　金：广东省佛山市卫计局科研课题项目;项目编号:2014161

摘　　要：目的探讨HCV-cAg和HCV-Ab两个指标联合检测对HCV感染的临床价值。方法对门诊和住院患者血液标本进行HCV-cAg、HCV-Ab及HCV-RNA联合检测,采用实时荧光定量PCR法检测HCV-RNA,采用酶联免疫吸附法(ELISA)检测试剂盒检测HCV-Ab和HCV-cAg。结果 HCV-Ab检测的灵敏度和特异度分别为90.91%和99.17%;HCV-cAg检测的灵敏度和特异度分别为70.25%和100%,两者的特异度相近,但是HCV-Ab检测的灵敏度比HCV-cAg的要高。两者联合检测时灵敏度和特异度分别为99.17%和99.17%,灵敏度明显增加。此外,HCV-Ab和HCV-cAg两者之间的检测结果无一致性。结论 HCV-Ab和HCV-cAg检测相互间无法替代,将抗-HCV和HCV-cAg两种指标结合起来检测,对临床上提高HCV感染的筛查和诊断具有重要的价值。Objective To explore the clinical value of combination of HCV-cAg and anti-HCV detection in HCV infection.Methods The serum samples from outpatients and inpatients were detected for HCV-cAg,anti-HCV and HCV-RNA. The quantitative real-time PCR was applied to detect HCV-RNA,and enzyme-linked immunosorbent assay（ELISA） kits were utilized to test anti-HCV and HCV-cAg）. Result The sensitivity and specificity of anti-HCV detection were 90.91% and 99.17% respectively,and that of HCV-cAg were 70.25% and 100% respectively. Notably,the sensitivity（99.17%） and specificity（99.17%） increased significantly in case of combinational detection method. In addition,no consistency between the results of anti-HCV and HCV-cAg was detected. Conclusion Combination detection of anti-HCV and HCV-cAg was recommended because of its remarkable advantage in screening and diagnosis of HIV infection.

关 键 词：HCV HCV-CAG HCV-AB 

分 类 号：R512.63[医药卫生—内科学] R446.62[医药卫生—临床医学]