注射型可吸收氨基酸聚合物/硫酸钙复合材料动物体内降解吸收及促成骨作用实验研究  

Experimental Study of in vivo Degradation, Absorption and Osteogenesis of Injected Absorbable Polyamine Acid/Calcium Sulfate Composites

作　　者：罗翼[1] 邹昌[1] 张帅[2] 周勇[1] 闵理[1] 张闻力[1] 石锐[1] 段宏[1] 屠重棋[1] 

机构地区：[1]四川大学华西医院骨科,成都610041 [2]第三军医大学西南医院骨科

出　　处：《华西医学》2015年第12期2224-2228,共5页West China Medical Journal

基　　金：四川省科技厅支撑计划(2010FZ0088)~~

摘　　要：目的观察注射型可吸收氨基酸聚合物/硫酸钙复合材料动物体内的促成骨作用,评估其修复骨缺损的能力。方法选取48只新西兰大白兔,雌雄各半,体质量2.0-2.5 kg。选择兔单侧股骨髁处钻孔制备骨缺损模型,将材料植入骨缺损处,以空白组作为对照,分别在术后4、8、12、16周处死动物取材,行X线摄片、硬组织切片组织学检查等,观察材料降解、吸收及成骨情况。结果实验组骨缺损孔道术后4周时X线片不显影,无明显骨痂生长;组织学可见材料开始降解,内部新生幼稚骨小梁。8周时X线片有少量骨痂生长;组织学可见材料继续降解,缺损内可见继续增多的排列不规则的新生骨小梁。12周时骨痂明显有生长,骨缺损区残留较小低密度影;组织学示材料大部分降解,部分区域编织骨开始转化为板层骨。16周时新骨长入,恢复正常松质骨密度;组织学可见材料基本完全降解,被新生骨替代,大部分新生骨已具有正常骨小梁结构。空白对照组骨缺损孔道术后12周内无明显变化,术后16周时部分骨缺损孔道变小,边缘可见部分修复。结论注射型可吸收氨基酸聚合物/硫酸钙复合材料在体内能够完全降解、吸收,具有良好的成骨活性,有望成为理想的骨修复材料。Objective To observe the ability of osteogenesis in vivo using the injected absorbable polyamine acid/calcium sulfate（PAA/CS） composites and assess their ability to repair bone defects. Methods We selected 48 New Zealand white rabbits, and half of them were male with a weight between 2.0 and 2.5 kg. Bone defect models were made at the rabbit femoral condyle using electric drill, and the rabbits were divided into two groups. One group accepted implantation of the material at the defect, while nothing was done for the control group. After four, eight, twelve and sixteen weeks, the animals were killed. h e line X-ray and hard tissue slices histological examination（HE, MASSON staining） were observed to assess the situation of degradation, absorption and bone formation of the material. Results Four weeks after operation, bone defect of the experimental group had no obvious callus growth on X-ray imaging. Histology showed that the material began to degrade and new immature trabecular bone grew. h e bone defect of the experimental group had a small amount of callus growth on X-ray imaging at er eight weeks. And histology showed that the material continued to degrade and new immature trabecular bone grew continually. h ere was an obvious callus growth at er twelve weeks, and the bone defect area had smaller residual low-density shadow on X-ray imaging. Histology showed that most of the materials degraded and parts of woven bone grew into lamellar bone. At er sixteen weeks, the

关 键 词：氨基酸聚合物 硫酸钙 复合材料 人工骨 成骨作用 实验研究 

分 类 号：R68[医药卫生—骨科学] R318.08[医药卫生—外科学]