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作 者:胡振帮[1] 刘航[1] 辛大伟[1] 蒋洪蔚[1,2] 朱荣胜[1] 陈庆山[1] 齐照明[1] 刘春燕[2]
机构地区:[1]东北农业大学大豆研究所/大豆生物学教育部重点实验室,黑龙江哈尔滨150030 [2]黑龙江省农垦科研育种中心,黑龙江哈尔滨150030
出 处:《大豆科学》2015年第6期945-949,共5页Soybean Science
基 金:教育部新世纪优秀人才支持计划(NECT-1207-01);国家自然基金面上项目(31271747;31471516);国家自然科学基金青年基金(31400074;31401465);黑龙江省自然科学基金重点项目(ZD201213);黑龙江省博士后基金(LBH-Z12035);中国博士后基金(2012M520030);黑龙江省高校长江后备支持计划项目(2014CJHB004)
摘 要:以野生大豆ZYD00006(父本)和绥农14(母本)为亲本构建的导入系为定位群体,群体含有102个株系,遗传图谱使用了329个SSR标记位点,针对粒长和粒宽2个籽粒性状进行定位分析。结果表明:有18个位点存在不同性状间被同时检测到的情况,其中与QTL连锁的位点Sat_227、Satt338和Satt568被2个性状同时检测到。通过比较分析也发现3个位点也同时影响着百粒重的大小,进一步通过加性效应分析表明,定位区段对粒长的影响为-10%~15.4%,定位区段对粒宽的影响为-26.72%~2.76%。明确导入位点对粒长和粒宽的影响,可以作为进一步大豆粒长和粒宽相关基因挖掘。Using the chromosome fragment introgression lines of soybean derived from the cross between ZYD00006( Glycine soja) as male parent and Suinong 14( Glycine max) as female parent,to study location of seed length and seed width. This population has 102 lines and 329 SSR markers were used to construct the genetic map. In the results,18 different traits were found underlying those two seed traits. And the QTL sites of Sat_227,Satt388 and Satt568 were found underlying those two traits at the same time. By the comparison,these 3 sites were found in different genetic population for seed traits. The additive effect of location interval underlying seed length was range from- 10% to 15. 4%,and the additive effect of location interval underlying seed width was range from- 26. 72% to 2. 76%. So these markers was useful to molecular assistant breeding and gene mining.
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