血管内皮生长因子对尾吊大鼠后肢胫骨骨丢失的影响  

Effects of vascular endothelial growth factor on bone loss of the proximal tibia in tail-suspended rats

作　　者：王宇星[1] 刘天亚[1] 樊瑜波[1,2] 

机构地区：[1]北京航空航天大学生物与医学工程学院,教育部生物力学与力生物学重点实验室,国际植介入医疗器械转化研究中心,北京100191 [2]国家康复辅具研究中心,北京100176

出　　处：《医用生物力学》2015年第6期547-552,共6页Journal of Medical Biomechanics

基　　金：国家重点基础研究发展计划(973)资助项目(2011CB710901);国家自然科学基金项目(11120101001;11421202)

摘　　要：目的探究血管内皮生长因子(vascular endothelial growth factor,VEGF)对废用性骨丢失的影响。方法建立尾吊大鼠的动物模型,并随机平均分为尾吊盐水组、尾吊VEGF组、对照盐水组、对照VEGF组。实验过程中,每周两次对大鼠右腓肠肌注射VEGF或者等量的生理盐水,4周后通过micro-CT对大鼠胫骨近端进行扫描,以松质骨和皮质骨骨密度(bone mineral density,BMD)、骨体积分数(BV/TV)、骨小梁数量(Tb.N)、骨小梁间距(Tb.Sp)和结构模型指数(SMI)等松质骨微结构参数以及皮质骨厚度作为评价指标,探讨VEGF对尾吊大鼠后肢胫骨骨丢失的影响。结果与对照组大鼠相比,尾吊组大鼠松质骨表观BMD、BV/TV、Tb.N以及皮质骨厚度均显著降低,Tb.Sp与SMI显著升高,尾吊会造成大鼠的骨丢失,而注射VEGF能够缓解尾吊大鼠松质骨的骨丢失。结论血供的改变可能与骨重建之间存在着一定的联系,改善血供有助于对抗废用性的骨丢失。Objective To investigate the effects of vascular endothelial growth factor （VEGF） on disused bone loss. Methods Tail-suspended （TS） rat models were established and evenly divided into TS＋Saline group, TS＋VEGF group, CON＋Saline group and CON＋VEGF group. During the experiment, VEGF or equivalent amount of saline was injected into the gastrocnemius muscle of the rats’ right tibia twice a week for each rat. After four weeks, the proximal tibia of the rats was scanned by micro-CT. The bone mineral density （BMD） of the trabecular bone and cortical bone, micro-structure parameters of the trabecular bone, such as bone volume per trabecular volume （BV/TV）, tabecular number （Tb.N）, trabecular spacing （Tb.Sp）, structure model index （SMI）, and cortical thickness were used as evaluation indices to study the influence of VEGF on bone loss in the proximal tibia of tail-suspended rats. Results Compared with the control rats, in tail-suspended groups, the apparent BMD, BV/TV, Tb.N, SMI of trabecular bone and the cortical thickness all decreased significantly, while the Tb.Sp and SMI significantly increased, which showed that tail suspension would cause bone loss, while the injection of VEGF would alleviate the loss of trabecular bone in tail-suspended rats. Conclusions There might exist some relationship between the status of blood supply and bone remodeling process, and by improving the status of vascular system, the disused bone loss can be alleviated.

关 键 词：血管内皮生长因子 骨丢失 尾吊 CT扫描 

分 类 号：R318.01[医药卫生—生物医学工程]