Simultaneous determination of naphazoline and pyridoxine in eye drops using excitation–emission matrix fluorescence coupled with second-order calibration method based on alternating trilinear decomposition algorithm  

作　　者：Hui Xia Hai-Long Wu Hui-Wen Gu Xiao-Li Yin Huan Fang Ru-Qin Yu 

机构地区：[1]State Key Laboratory of Chemo,Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

出　　处：《Chinese Chemical Letters》2015年第12期1446-1449,共4页中国化学快报（英文版）

基　　金：the financial supports from the National Natural Science Foundation of China(No.21175041);the National Basic Research Program of China(No.2012CB910602);the Foundation for Innovative Research Groups of NSFC(No.21221003)

摘　　要：A novel method is developed for the direct determination of naphazoline hydrochloride（NAP） and pyridoxine hydrochloride（VB6） in commercial eye drops. By using excitation–emission matrix（EEM）fluorescence coupled with second-order calibration method based on the alternating trilinear decomposition（ATLD） algorithm, the proposed approach can achieve quantitative analysis successfully even in the presence of unknown and uncalibrated interferences. The method shows good linearity for NAP and VB6 with correlation coefficients greater than 0.99. The results were in good agreement with the labeled contents. To further confirm the feasibility and reliability of the proposed method, the same batch samples were analyzed by multiple reaction monitoring（MRM） based on LC–MS/MS method.T-test demonstrated that there are no significant differences between the prediction results of the two methods. The satisfactory results obtained in this work indicate that the use of the second-order calibration method coupled with the EEM is a promising tool for industrial quality control and pharmaceutical analysis due to its advantages of high sensitivity, low-cost and simple implementation.A novel method is developed for the direct determination of naphazoline hydrochloride（NAP） and pyridoxine hydrochloride（VB6） in commercial eye drops. By using excitation–emission matrix（EEM）fluorescence coupled with second-order calibration method based on the alternating trilinear decomposition（ATLD） algorithm, the proposed approach can achieve quantitative analysis successfully even in the presence of unknown and uncalibrated interferences. The method shows good linearity for NAP and VB6 with correlation coefficients greater than 0.99. The results were in good agreement with the labeled contents. To further confirm the feasibility and reliability of the proposed method, the same batch samples were analyzed by multiple reaction monitoring（MRM） based on LC–MS/MS method.T-test demonstrated that there are no significant differences between the prediction results of the two methods. The satisfactory results obtained in this work indicate that the use of the second-order calibration method coupled with the EEM is a promising tool for industrial quality control and pharmaceutical analysis due to its advantages of high sensitivity, low-cost and simple implementation.

关 键 词：calibration excitation satisfactory labeled interpolation pharmaceutical normalized alternating batch scatter 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]