Isolation of hemoglobin with metal–organic frameworks Y(BTC)(H_2O)_6  

作　　者：Yang Shu Ying Meng Ming-Li Chen Jian-Hua Wang 

机构地区：[1]Research Center for Analytical Sciences, College of Sciences,Northeastern University [2]Institute of Biological Technology, College of Life and Health Sciences, Northeastern University

出　　处：《Chinese Chemical Letters》2015年第12期1460-1464,共5页中国化学快报（英文版）

基　　金：financial supports from the National Natural Science Foundation of China(Nos.21235001,21375013,21475017,21575020);Fundamental Research Funds for the Central Universities(Nos.N140505003;N141008001)

摘　　要：The hierarchical metal-organic frameworks（MOFs）,such as Y（BTC）（H_2O）_6,are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature.The product is characterized by Fourier transform infrared（FT-IR）,X-ray diffraction（XRD）,scanning electron microscopy（SEM）and thermogravimetric analysis（TGA）.The Y（BTC）（H_2O）_6 particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin.The adsorption behavior of hemoglobin onto the Y（BTC）（H_2O）_6 fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g 1.An adsorption efficiency of 87.7%for 100μg mL 1hemoglobin in 1 mL sample solution（at pH 6.0）is achieved with 0.40 mg Y（BTC）（H20）6.77.3%of the retained hemoglobin is readily recovered using a 0.5%（m/v）SDS solution as the stripping reagent.Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption-desorption process.The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）.The hierarchical metal-organic frameworks（MOFs）,such as Y（BTC）（H_2O）_6,are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature.The product is characterized by Fourier transform infrared（FT-IR）,X-ray diffraction（XRD）,scanning electron microscopy（SEM）and thermogravimetric analysis（TGA）.The Y（BTC）（H_2O）_6 particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin.The adsorption behavior of hemoglobin onto the Y（BTC）（H_2O）_6 fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g 1.An adsorption efficiency of 87.7%for 100μg mL 1hemoglobin in 1 mL sample solution（at pH 6.0）is achieved with 0.40 mg Y（BTC）（H20）6.77.3%of the retained hemoglobin is readily recovered using a 0.5%（m/v）SDS solution as the stripping reagent.Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption-desorption process.The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）.

关 键 词：hemoglobin BTC H2O hierarchical selectivity nitrate desorption verified dodecyl polyacrylamide 

分 类 号：Q51[生物学—生物化学]