HPLC-ESI-MS联用法分析血燕与白燕水解多肽研究  

Study on Hydrolyzed Peptides of Red Cubilose and White Cubilose by HPLC-ESI-MS

作　　者：李耿[1] 戴洁[1] 赵冉[1] 赵斌[1] 彭敏桦 赖小平[2] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,东莞523000

出　　处：《世界科学技术-中医药现代化》2015年第11期2202-2208,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81173498):特异性DNA探针和特征肽指纹图谱技术在燕窝质量评价中的应用;负责人:赖小平;科学技术部港澳台科技合作专项项目(2014DFH30010):粤港澳岭南中药综合开发研究;负责人:赖小平

摘　　要：目的:应用高效液相色谱-电喷雾质谱联用技术,对血燕与白燕的水解多肽进行测定,比较不同种类燕窝水解多肽一级结构的异同。方法:运用SDS-PAGE、毛细管高效液相色谱、ESI质谱法对样品进行纯度检查和肽图谱测定,通过Mascot 2.2软件进行数据库检索。结果:质谱分析Basepeak图显示,血燕与白燕的水解多肽具有较好的一致性。利用Mascot 2.2软件数据库对下载于Uniprot的禽类数据库进行检索,鉴定白燕中73种蛋白,鉴定血燕中51种蛋白,发现血燕和白燕有30种相同蛋白。结论:血燕与白燕水解肽谱有较高的相似度,血燕与白燕的共有蛋白可作为燕窝产品的质量控制特征。HPLC-ESI-MS technology was applied to determine the hydrolyzed peptides of red cubilose and white Abscubilose for the comparison of similarities and differences between different kinds of cubiloses＇ hydrolysis polypeptide primary structure. SDS-PAGE, capillary high-performance liquid chromatography and ESI-MS were used to detect the purity and peptide mapping of samples. Mascot 2.2 software was used to search the database. The basepeak figure in mass spectrometry analysis showed good consistency of hydrolyzed peptides between red cubilose and white cubilose. The Mascot 2.2 software was used to search the poultry database which had been downloaded from the Uni Prot. A total of 73 kinds of proteins from white cubilose were identified. And a total of 51 kinds of proteins from red cubilose were identified. There were 30 kinds of same proteins from both cubilose. It was concluded that the hydrolyzed peptides had relatively high similarity between red cubilose and white cubilose. The same proteins from both red and white cubilose can be used as the quality control characteristics of cubilose products.

关 键 词：血燕 白燕 肽指纹图谱 毛细管高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]