Box-Behnken设计-效应面法优化黄连多糖的提取工艺  

Optimization on Extraction Technology of Polysaccharide from Rhizoma Coptidis by Box-Behnken Design-Response Surface Methodology

作　　者：吕秀梅[1] 唐策[1] 范刚[1] 李艳[1] 张静[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137

出　　处：《世界科学技术-中医药现代化》2015年第11期2240-2245,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：四川省教育厅2013年科研计划项目(13ZB0319):中药黄连多糖的化学表征及其与生物碱类组分协同降血糖活性研究;负责人:范刚;成都中医药大学科技发展基金(ZRMS201342):黄连药材代谢物组"产地趋向变异"规律及道地性特征markers研究;负责人:范刚;四川省杰出青年学术技术带头人培育计划项目(2014JQ0050):"上火"的生物学特征及三黄泻心汤"清热泻火"物质基础和作用机理研究;负责人:范刚

摘　　要：目的:本研究旨在优选黄连中黄连多糖的提取工艺条件。方法:采用Box-Behnken设计-效应面法,以黄连出膏率、多糖得率和糖醛酸得率为评价指标,考察黄连多糖回流提取过程的提取温度、提取次数、提取时间和料液比对提取工艺的影响。结果:最终确定黄连多糖的最佳提取条件为:提取温度100℃,提取时间3.8 h,提取次数3次,料液比1?15.7。结论:运用Box-Behnken设计-效应面法优化黄连多糖的提取方法是准确、合理、可行的。This study was aimed to optimize the extraction conditions of polysaccharides from Rhizoma Coptidis. With the R. Coptidis of extraction yield, polysaccharide yield and uronic acid yield as evaluation indexes, the impact of extraction temperature, extraction times, extraction duration and liquid-to-solid ratio on the process of R. Coptidis polysaccharides reflux extraction were investigated by the Box-Behnken design-response surface methodology. The results showed that the optimal extraction conditions were achieved and listed as follows： extraction temperature at 100℃, extracted 3 times with 3.8 h per each time, liquid-to-solid ratio of 1：15.7. It was concluded that the Box-Behnken design-response surface methodology was accurate, rational and feasible to optimize the extraction method of polysaccharides from R. Coptidis.

关 键 词：黄连 多糖 Box-Behnken设计-响应面法 提取工艺 

分 类 号：R284[医药卫生—中药学]