白芍提取物对经前期综合征肝气郁证模型大鼠额叶5-HT_(3A/3B)R分布与蛋白表达的影响  

Effects of Radix Paeoniae Alba Extract on Distribution and Expression of 5-HT_(3A/3B) R in Frontal Lobe of Premenstrual Syndrome Rat Model with Liver-qi Depression Pattern

作　　者：李芳[1] 宋春红[2] 魏盛[2] 张惠云[2] 乔明琦[2] 

机构地区：[1]北京市丰台区妇幼保健院药剂科,北京100067 [2]山东中医药大学中医药经典理论教育部重点实验室,济南250355

出　　处：《世界科学技术-中医药现代化》2015年第11期2267-2271,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81173163):从谷氨酸-γ氨基丁酸代谢通路探讨经前期综合征肝气逆;郁两证发病机制;负责人:张惠云;国家自然科学基金委面上项目(81173151):雌激素受体β介导的中枢5-HT功能在经前期综合征肝气逆郁两证中的作用;负责人:乔明琦

摘　　要：目的:本研究就5-羟色胺3受体(5-HT3R)水平探讨白芍提取物(Radix Paeoniae Alba Extract,RPAE)干预经前期综合征(Premenstrual Syndrome,PMS)肝气郁证抑郁情绪的分子作用机制。方法:复制PMS肝气郁证大鼠模型,用白芍提取物进行药物干预,采用免疫荧光技术和蛋白印迹技术(western blot)分别检测5-HT3AR和5-HT_(3B)R在大鼠脑内的分布和蛋白表达情况。结果 :5-HT_(3A)R和5-HT_(3B)R在各组大鼠额叶均有阳性表达;与正常组大鼠相比,模型组大鼠额叶5-HT3AR、5-HT_(3B)R蛋白表达水平均显著升高(P<0.05);与模型组大鼠相比,白芍提取物组大鼠额叶5-HT_(3A)R蛋白表达水平均显著下降(P<0.05)。结论:白芍提取物能够调控PMS抑郁症大鼠额叶5-HT_(3A) R和5-HT_(3B)R蛋白表达,这可能是其干预PMS肝气郁证抑郁情绪的作用机制之一。This study was aimed to elucidate the 5-HT_3 R molecular mechanism of Radix Paeoniae Alba extract（RPAE） as drug intervention in the treatment of premenstrual syndrome（PMS） model rats with liver-qi depression pattern. PMS model rats with liver-qi depression pattern were prepared. And then, the model was treated with RPAE. The protein distribution of 5-HT_（3A）R and 5-HT_（3B）R in the frontal lobe was evaluated by the immune fluorescence technology and western blot. The results showed that there were positive expressions of 5-HT_（3A）R and 5-HT_（3B）R in frontal lobe of rats in each group. Compared with the normal group, the 5-HT_（3A）R and 5-HT_（3B）R protein expression levels of the frontal lobe in the model group increased significantly（P〈0.05）. Compared with the model group, the 5-HT_（3A）R protein expression level in the frontal lobe decreased significantly after RPAE treatment（P〈0.05）. In conclusion, RPAE regulated the protein expressions of 5-HT_（3A）R and 5-HT_（3B）R in frontal lobe, which may be one of the mechanisms for its treatment of PMS with liver-qi depression pattern.

关 键 词：白芍提取物 经前期综合征 肝气郁证 额叶 5-HT3AR 5-HT3BR 蛋白表达 

分 类 号：R284[医药卫生—中药学]