Box-Behnken响应面法优化疏风定喘颗粒黄芩组的提取工艺研究  

Optimization on Extraction Technology of Shu-Feng Ding-Chuan Granules in Huang-Qin Group by Box-Behnken Response Surface Methodology

作　　者：李锐华[1,2] 李英[2,3] 王永香[2,3] 吴云[2,3] 丁岗[1,2,3] 

机构地区：[1]南京中医药大学药学院,南京210000 [2]江苏康缘药业股份有限公司,连云港222001 [3]中药制药过程新技术国家重点实验室,连云港222001

出　　处：《世界科学技术-中医药现代化》2015年第11期2290-2295,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重大新药创制项目(2015ZX09101043-003):现代中药创新集群与数字制药技术平台;负责人:王振中

摘　　要：目的:利用Box-Behnken响应面法对疏风定喘颗粒黄芩组的提取工艺进行优化。方法:以黄芩苷、白花前胡甲素和固含转移率为考察指标,在单因素试验的基础上,考察乙醇浓度、乙醇用量、提取时间、提取次数4个因素对提取工艺的影响,再结合Box-Behnken中心组合设计试验,进一步优化醇提工艺参数。结果:单因素试验结合Box-Behnken响应面法优选黄芩组的最佳工艺为:8倍量70%乙醇,提取两次,每次1.0 h,在此条件下黄芩苷、白花前胡甲素的转移率分别达到89.57%、87.90%,固含转移率为32.35%。结论:单因素试验结合响应面法可用于优化疏风定喘颗粒黄芩组的提取工艺,该工艺稳定可行,为工业化生产提供科学的依据。This study was aimed to optimize the extraction technology of Shu-Feng Ding-Chuan（SFDC） granules in the Huang-Qin（HQ） group by Box-Behnken response surface methodology（RSM）. With three major characteristic components（baicalin, praeruptorin A and solid content in extraction liquid）, the effects of four factors, such as the concentration of ethanol, the dosage of ethanol, the duration of extraction and the extraction times, were investigated by the single factor experiment. Then, the range of parameters of key factors was further studied and explored by Box-Behnken RSM. The results of single factor experiment and Box-Behnken design showed that the optimum preparation conditions were 8-fold 70% ethanol, extracted for 2 times, with 1.0 h per each time. Under these conditions, the transfer rates of baicalin and praeruptorin A were 89.57% and 87.90%, respectively. And the transfer rate of solid content was 32.35%. It was concluded that the single factor experiment combined with RSM can be used in the optimization of extraction technology for SFDC granules in the HQ group. This technique was stable and feasible, which provided scientific evidences for the industrial production.

关 键 词：疏风定喘颗粒 响应面法 提取工艺 单因素试验 黄芩苷 白花前胡甲素 

分 类 号：R284.2[医药卫生—中药学]