流式微球术、胶体金渗滤法及ELISA法检测HIV抗体的评价与分析  

Evaluation and Analysis of HIV Antibodies Detected with Flow Microbeads Assay,Dot Immune-gold Filtration Assay and ELISA

作　　者：乔军 刘俊娜 王春娟 张洁[2] 

机构地区：[1]西安市长安区医院,西安710100 [2]陕西省人民医院,西安710068

出　　处：《现代检验医学杂志》2015年第6期104-104,108,共2页Journal of Modern Laboratory Medicine

摘　　要：目的探讨采用流式微球术（FMA），胶体金斑点免疫渗滤法（DIGFA）与ELISA法检测HIV抗体三种试验之关系，为提高艾滋病检测技术提供依据。方法分别采用FMA，DIGFA与ELISA法检测HIV抗体阳性血清标本98例和86例HIV抗体阴性标本。结果FMA与ELISA的K一致性检验，k值=0．9112，P〈0．01，提示两种方法的检测结果具有极强的一致性。FMA与DIGFA的K一致性检验，k值=0．9032，P〈0．01，提示两种方法的检测结果具有极强的一致性。故三种方法的检测结果具有极强的一致性。FMA法检测阳性率为100％，而DIGFA检测阳性率是94‰。ELISA法检测阳性率为95％。结论三种试验方法检测的特异度和灵敏度没有显著性差异。Objective To explore the relation among adopting FMA,the colloidal gold osmosis of immune systern spots and detecting all HIV antibodies in order to improve HIV testing technology. Methods Adopted three different methods,FMA, the osmosis of immune systern spots of colloidal gold and detecting all HIV antibodies to test 98 positive serum samples and 86 negative serum samples. Results The detection of K consistency of FMA and ELISA was：k=0. 911 2,P〈0. 01. Using the two methds, found that the two kinds of test results were extremely consistent. The detection result of K consistency of FMA and DIGFA was： k = 0. 903 2, P〈0.01 which shows that test results were extremely consistent. So three methods could lead to the extreme consistency. By using FAM could assure that the positive rate of detection was 100%. By using the colloidal gold osmosis, found that the positive rate of detection was 94%. By using EI.ISA the positive rate was 95%. Conclusion There were no significant differences in detecting the specificity and sensitivity.

关 键 词：HIV抗体 流式微球术 胶体金渗滤法 酶联免疫吸附试验 

分 类 号：R446.61[医药卫生—诊断学]