水稻根系响应镉胁迫的蛋白质差异表达  

Analysis of the differently expressed proteins in rice roots in response to cadmium stress

作　　者：肖清铁[1] 王经源[1] 郑新宇[1] 戎红[1] 张国君[1] 王良华[1] 谢惠玲[1] 李艺[1] 陈珊[1] 林瑞余[1] 林文雄[1] 

机构地区：[1]福建农林大学生命科学学院农业生态研究所,福州350002

出　　处：《生态学报》2015年第24期8276-8283,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(31070403);福建省自然科学基金项目(2009J01056;2013J01083);福建省教育厅基金项目(JA09084);福建省高校服务海西建设重点项目(0B08B005);福建农林大学重点项目建设专项(6112c0604)

摘　　要：为探讨水稻根系对镉胁迫的分子生理响应,以抗镉水稻PI312777和镉敏感水稻IR24为材料,设置Cd^(2+)浓度为0、50和100μmol/L的水培试验,处理7 d后分析了水稻根系的蛋白质差异表达。结果表明,在镉胁迫下水稻PI312777和IR24根系有18个蛋白质发生了差异表达,其中的12个得到MALDI-TOF/MS鉴定。这些鉴定的蛋白功能可分四类:(1)与活性氧(ROS)胁迫相关的过氧化物酶(POD)、蛋氨酸腺苷转移酶(MAT)、类萌发素蛋白前体;(2)与谷胱甘肽(GSH)合成相关的S-腺苷甲硫氨酸合成酶(SAMS)、谷氨酰胺合成酶(GS)和谷氨酸脱氢酶(GDH);(3)与逆境胁迫相关的ABA胁迫诱导蛋白含HVA22域蛋白、ABA-胁迫-成熟诱导蛋白5(ASR5);(4)与细胞分裂调控相关的GTP结合核蛋白Ran-2。镉胁迫下SAMS和GTP结合核蛋白Ran-2在两种水稻根系均发生上调表达;MAT、POD、类萌发素蛋白前体和GS发生下调表达;依赖NADP-GDH、GDH和磷酸甘油酸变位酶在IR24根部均发生下调表达,在PI312777根部仅在100μmol/L Cd^(2+)处理发生下调表达;含HVA22域蛋白在PI312777根部上调表达,在IR24根部发生下调表达;ASR5在PI312777根部上调表达,在IR24根部的表达无显著差异;100μmol/L Cd^(2+)胁迫下60S酸性核糖体蛋白P0在水稻PI312777根部表达下调,在IR24根部表达上调。可见,镉胁迫使水稻根部ROS增加,形成氧化胁迫反应,造成毒害作用,而水稻根通过调节SAMS和GS提高GSH合成降低镉毒害。ASR5和HVA22蛋白等逆境胁迫蛋白的表达差异则是水稻品种间抗性差异的重要原因之一。To investigate the molecular and physiological responses in rice roots to cadmium （Cd） stress, a set of hydroponic culture experiments treated with Cd2＋at0, 50, and 100 p, mol/L in the solutions were conducted to analyze the differentially expressed proteins in the roots of two rice （ Oryza satiwz L. ） cultivars （ Cd-tolerant rice PI312777 and Cdsensitive rice IR24） 7 days after the treatments. The results showed that 18 proteins were differentially expressed in the roots of PI312777 and IR24 under Cd stress; 12 of them were identified by the MALDI-TOF/MS technique. The biological functions of these identified proteins were involved in four metabolic pathways, including （1） reactive oxygen species （ROS） stress ： peroxidase （ POD ） , methionine adenosyltransferase （MAT） , and germin-like protein precursor; （ 2 ） glutathione （GSH） synthesis： S-adenosylmethionine synthetase （SAMS）, glutamine synthetase （GS）, and glutamate dehydrogenase （GDH） ; （3） stress response induced by abscisic acid （ABA） ： HVA22 and abscisic acid-stress-ripeninginducible 5 protein （ASR5） ; and （d） cell division regulation： GTP-binding nuclear protein Ran-2 Under Cd stress conditions, the expression of SAMS and GTP-binding nuclear protein Ran-2 were up-regnlated and MAT, POD, the germin-like protein precursor, and GS were down-regulated in the roots of the two rice cultivars. The expression of NADP-GDH, GDH and phosphoglycerate mutase were down-regulated in the roots of rice IR24, but they were down-regulated in PI312777 only upon the treatment with 100 ~mol/L Cd2~. The expression of protein with HVA22 domain was up-regulated in PI312777, but it was downregulated reversed in IR24. ASR5 was up-regulated in rice PI312777, but no significant change was found in IR24. The 60s acidic ribosomal protein P0 was down-regulated in PI312777, but upregulated in IR24 at 100 μmol/L Cd2＋condition. Our results suggest that Cd stress increases ROS and produces oxidative stress in rice

关 键 词：水稻 根系 镉 胁迫反应 蛋白质组学 

分 类 号：S511[农业科学—作物学]