西藏小型猪慢性肺动脉高压动物模型的建立  

Establishment of Chronic Pulmonary Hypertension Model in Tibet Minipig

作　　者：刘蓉[1,2] 侯梦[1,2] 廖海星[1] 张新凤[1] 赵瑾[1,2] 刘宝骅[1] 钱元新[1] 吴清洪[3] 莫自耀[1,2] 廖东江[1,2] 李洪涛[1,2] 顾为望[3] 

机构地区：[1]广州医科大学附属第一医院,广州510120 [2]广州医科大学呼吸疾病国家重点实验室,广州510230 [3]南方医科大学实验动物中心,广州510515

出　　处：《实验动物与比较医学》2015年第6期431-435,共5页Laboratory Animal and Comparative Medicine

基　　金：【基金项目】国家973项目（2011CBA01006）;广东省科研基础条件建设项目（20128060300032）

摘　　要：目的采用野百合碱（MCT）诱导方法建立幼龄西藏小型猪慢性肺动脉高压（PH）动物模型，并通过右心导管术和肺脏结构形态进行评价。方法2月龄西藏小型猪12头，体质量5．0-6．0kg。随机均分为实验组（MCT组）和对照组，分别腹腔注射给予MCT12．0mg／kg和同样剂量的溶剂（体积分数75％乙醇）；6周后采用右心导管术测定小型猪肺动脉压；观察小型猪右心肥大指数和肺组织病理改变。结果与对照组相比，MCT组西藏小型猪平均肺动脉压显著增加（24．62±1．38mmHgVS15．10±0．76mmHg，P〈0．01）。肺组织有明显血管堵塞、血管腔消失，也可见肺小血管重构，有肺小动脉闭塞、向心性内膜增厚（丛状血管）的改变。实验组小型猪右心肥大指数增加明显（O．36±0．16VS0．28±0．13，P〈0．01）。结论通过腹腔注射MCT6周后，可以建立西藏小型猪慢性PH动物模型，其病理特征与人类PH临床指标相似。Objectives To establish chronic pulmonary hypertension （PH） model with monocrotaline （MCT） in young Tibet minipigs. Methods Twelve Tibet minipigs （weighing 5.0~6.0 kg, aged 2.0 months） were randomly divided into two groups and treated with MCT （12.0 mg/kg） or placebo （75% ethanol）. After six weeks, the pulmonary arterial pressures of the minipigs were measured by performing right heart catheterization （RHC）. The pathologic changes of small pulmonary arteries were observed. Index of right ventricular hypertrophy was also compared between two groups. Results The mean pulmo- nary arterial pressure by RHC was obviously increased in the MCT group than that in control group （24.62 ± 1.38 mmHg vs 15.10 ± 0.76 mmHg, P〈0.01）. The pathologic observations showed that.the pulmonary vascular remodeling and the lumen disappeared in small pulmonary arteries. There,was also progressive intimal fibrosis accompany with hyalinization, the lumen was occlusive, twisty and distorted with plexiform lesions. Indexes of right ventricular hypertrophy of MCT group were significantly higher （0.36 ± 0.16 vs 0.28 ± 0.13, P〈0.05）. Conclusions An animal model of chronic PH was established successfully in young minipigs at six weeks after MCT was intraperitoneally injected. The features of the chronic PH model of minipigs are similar with the primary PH parameters.

关 键 词：肺动脉高压(PH) 野百合碱(MCT) 西藏小型猪 右心导管术 

分 类 号：R544.1[医药卫生—心血管疾病] R-332[医药卫生—内科学]