子痫前期大鼠模型建立及相关指标监测  

Establishment of Preeclampsia Model in Rat and Detection on Related Indicators

作　　者：周晶[1,2] 严国锋[1] 罗章源 陈东宝[1] 陈学进[1] 李垚[1] 

机构地区：[1]上海交通大学医学院实验动物科学部,上海200025 [2]复旦大学生命科学学院,上海200433 [3]杰升生物科技(上海)有限公司,上海201112

出　　处：《实验动物与比较医学》2015年第6期448-452,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的使用SD大鼠构建子痫前期动物模型，并采用遥测法监测模型大鼠的动脉血压，分析掌握模型动物在自由活动状态下的血压及其他各项生理生化指标。方法将16只SD大鼠分为4组，遥测模型组A：大鼠植入遥测子并构建子痫前期模型。模型组B：仅构建子痫前期模型。遥测对照组C：植入遥测子并制作模型假手术。对照组D：仅制作子痫前期假手术。A、c组在遥测子植入后连续监测血压数据。各组收集妊娠13．5d、20．5d尿液检测尿总蛋白、尿肌酐。妊娠21．5d临产前将母鼠安乐死，取肝、肾、胎盘组织进行HE染色。结果模型组A制模2d后平均动脉压、收缩压、舒张压较对照组C显著上升（P〈0．01），妊娠21．5d时平均动脉压、收缩压、舒张压较对照组C显著降低（P〈0．01）。模型组A、B尿总蛋白／肌酐比值显著高于对照组C、D（P〈0．01），同时A、B两组肝、肾、胎盘组织结构也出现了改变，并且出现70．49％胎鼠发育停止。结论成功构建子痫前期大鼠模型，获得大鼠在自由活动状态下的动脉压、尿生化、胎盘胎鼠发育情况，掌握了模型动物在妊娠期真实的、连续的血压变化周期。该模型或可成为子痫前期疾病研究的理想动物模型。Objective To establish preeclampsia SD rat model and monitor the alterations of related indicators in conscious rats. Methods Sixteen SD rats were divided into 4 groups randomly. Telemetrical modeling group A, which was implanted radiotelemetry and built preeclampsia model. Group B was modeling control group, which only built preeclampsia model without radiotelemetry implantation. Group C was implanted radiotelemetry and operated sham-operation of preeclampsia modeling. Group D was only operated sham-operation of preeclampsia modeling. Blood pressure of rats in groups A and C were monitored continuously after radiotelemetry implantation. Urine samples were collected from each group at pregnant day 13.5 and day 20.5 for urine TP and creatinine test. Pregnant rats were euthanieed at day 21.5 of pregnancy, and then the liver, kidney and placenta tissues were Collected for HE staining. Results Mean arterial pressure, systolic pressure and diastolic pressure of group A were significant higher than those of group C （P〈0.01） after modeling 2 days, but at the day 21.5 of pregnancy, mean arterial pressure, systolic pressure and diastolic pressure of group A were significantly decreased compared to group C （P〈0.01）. In groups A and B urine TP/creatinine ratio were significantly higher than those of groups C and D（P〈0.01）, also their liver, kidney and placenta tissue structure get pathological changes, and 70.49% fetal growth were ceased in groups A and B. Conclusion Preeclampsia model rats have been established successfully. This rat modeel will contribute to research of preeclampsia.

关 键 词：生理遥测 子宫胎盘缺血再灌注模型(RUPP) 子痫前期(PE) 平均动脉压(MAP) 

分 类 号：R714.244[医药卫生—妇产科学] R-332[医药卫生—临床医学]