孕中期限饲法建立胎儿宫内生长受限小鼠模型  

Mouse Model for Fetal Growth Restriction in Pregnant Metaphase by Limited Feeding

作　　者：刘学旭[1] 杨文[1] 丁运萍[2] 

机构地区：[1]四川大学实验动物中心,成都610041 [2]四川大学口腔疾病研究国家重点实验室,成都610041

出　　处：《实验动物与比较医学》2015年第6期453-457,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的研究孕中期限饲法建立胎儿宫内生长受I％（FGR）模型的可行性。方法将90只（雌性60只，雄性30只）3月龄KM小鼠随机分为对照组，模型Ⅰ、Ⅱ、Ⅲ和Ⅳ组，每组雌12只、雄6只，雌雄2：1合笼。所有孕鼠在妊娠10d前自由采食，从妊娠10d，模型Ⅰ、Ⅱ、Ⅲ和Ⅳ组组分别给予对照组相对应孕期80％、70％、60％和50％的日粮，对照组自由采食。结果对照组、模型Ⅰ、Ⅱ、Ⅲ和Ⅳ组：妊娠15d孕鼠体质量分别平均增长26．03g、21．31g、19．09g、16．96g和13．19g，模型组孕鼠体质量增长均低于对照组（模型Ⅱ组P〈0．05、模型Ⅲ组和Ⅳ组P〈0．01）：平均窝重分别是21．12g、19．20g、18．95g、15．59g和12．99g，模型组胎仔平均窝重低于对照组（模型Ⅲ组P〈0．05、Ⅳ组P〈0．01）；模型Ⅰ、Ⅱ、Ⅲ和Ⅳ组组FGR发生率分别为42．97％、66．17％、66．38％和54．26％，与对照组（4．76％）比较显著增高（P〈O．01）；模型组新生胎儿脏器平均质量除脑组织、模型Iv组的心脏、肝脏外均明显低于对照组（P〈0．05）。结论孕中期限饲法建立胎儿宫内生长受限动物模型是可行的，其中给予正常孕鼠同期60％～70％的日粮建立FGR模型效果较佳。Objective To established the fetal growth restriction （FGR） mouse model in pregnant metaphase by limited feeding. Methods Ninety KM mice （60 females and 30 males, 3 months old） were divided randomly into 5 groups consisting of the control, model Ⅰ, Ⅱ, Ⅲ and Ⅳ group. Mice in each group （12 female and 6 male） were placed in the mating cage with one male and 2 females. All the pregnant mice were fed ad libitum before day 10 of pregnancy. After that, model Ⅰ, Ⅱ, Ⅲ and Ⅳ group were fed with 80%, 70%, 60% and 50% of daily ration respectively according to control group feeding ad libitum. Results The average weight increment in 15-day pregnancy mice was 26.03 g, 21.31 g, 19.09 g, 16.96 g and 13.19 g in control, model Ⅰ, Ⅱ, Ⅲ and Ⅳ group, respectively. The weight increment in model group was significantly higher than that of control group （model II group P〈0.05, III group and IV group P〈0.01）; The average litter weight was 21.12 g, 19.20 g, 18.95 g, 15.59 g and 12.99 g in control, model Ⅰ, Ⅱ, Ⅲ and Ⅳ group, respectively. The litter weight in model group was significantly lower than that of control group （model Ⅲ group P〈0.05, Ⅲ group P〈0.01）; The incidence of FGR in model Ⅰ, Ⅱ, Ⅲ Ⅳ and control group was 42.97%, 66.17%, 66.38%, 54.26% and 4.76%, respectively. The incidence in all model groups was significantly higher than that in control group （P〈0.01）. Except for the brain tissue in all model groups as well as the heart and liver tissue in model IV group, all the fetal organ weight in model group was significantly lower than that in control group （P〈0.05）. Conclusion It is feasible to establish the FGR model in pregnant metaphase by limited feeding. The best FGR model may be obtained by feeding the pregnant mice at 70% to 60% of daily ration of normal pregnant mice.

关 键 词：胎儿宫内生长受限(FGR) 限饲法 动物模型 KM小鼠 

分 类 号：R714.5[医药卫生—妇产科学] R-332[医药卫生—临床医学]