电针“内关”穴对心肌缺血大鼠心肌氯离子通道相关基因表达的影响  

Effect of Electroacupuncture Stimulation of “Neiguan”(PC 6)on Expression of Myocardial Chloride Channel-related Genes,PKC and PKG Proteins in Myocardial Ischemia Rats

作　　者：白增华[1] 吴兆利[1] 苏妆[1] 丛培玮[2] 陈以国[1] 李春日[1] 武林[1] 

机构地区：[1]辽宁中医药大学针灸推拿学院,沈阳110847 [2]辽宁中医药大学实验中心,沈阳110087

出　　处：《针刺研究》2015年第6期439-443,共5页Acupuncture Research

基　　金：国家重点基础研究发展计划("973"计划)资助项目(No.2012CB 518503)

摘　　要：目的:观察电针"内关"穴对心肌缺血大鼠心肌氯离子通道调控基因表达的影响,探讨经穴效应特异性的作用机制。方法:SD大鼠随机分为正常对照组10只,模型组、内关穴组、列缺穴组、非经非穴组各15只。皮下注射异丙肾上腺素建立心肌缺血模型。内关穴组取"内关"穴,列缺穴组取"列缺"穴、非经非穴组取"天枢"与"神阙"连线中点进行电针治疗,每日治疗1次,治疗7d。Western blot法和Real time-PCR法分别检测心肌组织蛋白激酶C(PKC)和氯离子通道基因囊性纤维化跨膜传导调节因子(CFTR)及钙激活氯通道(CLCa 1)的基因表达。结果:与正常对照组比较,模型组大鼠心肌PKC、CLCa l、CFTR表达升高(P<0.05)。与模型组比较,内关穴组、列缺穴组及非经非穴组大鼠心肌PKC表达明显下降(P<0.05),内关穴组和列缺穴组与非经非穴组比较PKC表达下降(P<0.05)。与模型组比较,内关穴组、列缺穴组及非经非穴组心肌CLCa l基因表达显著降低(P<0.05),列缺穴组、非经非穴组与内关穴组比较显著升高(P<0.05),列缺穴组与非经非穴组比较显著下降(P<0.05)。与模型组比较,内关穴组、列缺穴组CFTR基因表达显著下降(P<0.05),列缺穴组、非经非穴组与内关穴组比较显著升高(P<0.05),而列缺穴组与非经非穴组比较显著下降(P<0.05)。结论:电针不同穴位对心肌缺血大鼠心肌PKC、CFTR、CLCa 1的表达有不同的影响,"内关"穴具有特异性效应。Objective To observe the effect of electroacupuncture（EA）stimulation of＂Neiguan＂（PC 6）,etc.on expression levels of myocardial chloride（CL-）channel-related genes and intracellular protein kinase C（PKC）protein in myocardial ischemia（MI）rats.Methods Seventy SD rats were randomly divided into control group（n=10）,model group（n=15）,Neiguan（PC 6）group（n=15）,Lieque（LU 7）group（n=15）and non-acupoint group（n=15）.The MI model was established by i.p.of isoproterenol（ISO,a sympathomimetic beta adrenergic agonist）.Electroacupuncture stimulation was applied to bilateral＂Neiguan＂（PC 6）,＂Lieque＂（LU 7）,or non-acupoint[the mid-point between＂Tianshu＂（ST 25）and＂Shenque＂（CV 8）]for 15 min,once a day for 7days.Quantitative RT-PCR was employed to detect the expression levels of cystic fibrosis transmembrane conductance regulator（CFTR,a CL-channel）mRNA and chloride channel calcium-activated 1（CLCa 1,a member of the family of calcium-activated chloride channels,CLCa）mRNA in the left cardiac ventricle tissue,and Western blot was used to detect the expression level of myocardial PKC protein of the left ventricle.Results Compared with the control group,the expression levels of myocardial PKC protein,and CLCa 1and CFTR genes were significantly increased in the model group（P〈0.05）.In comparison with the model group,the expression levels of myocardial PKC protein,and CFTR mRNA and CLCa 1mRNA in the Neiguan group,and PKC protein and CLCa 1mRNA in the Lieque and non-acupoint groups,as well as CFTR mRNA in the Lieque group were notably down-regulated（P〈0.05）.No significant change was found in the expression of CFTR mRNA in the non-acupoint group（P〈0.05）,and no significant differences were found between Neiguan and Lieque groups in the expression levels of PKC protein（P〈0.05）.The effects of＂Neiguan＂（PC 6）were obviously superior to those of non-acupoint in down-regulating myocardial PKC protein,CLCa 1mRNA and CFTR mRNA（P〈

关 键 词：心肌缺血 电针 内关 左心室 囊性纤维化跨膜传导调节因子基因 钙激活氯通道基因 

分 类 号：R245.917[医药卫生—针灸推拿学]