巴马小型猪1型糖尿病模型的建立  

Establishment of Type 1 Diabetes Mellitus Model in Bama Minipig

作　　者：刘亚千[1] 赵玉琼[1] 王凯[1] 相磊[1] 张琪[1] 谭清波[1] 杨述林[2] 陈华[1] 

机构地区：[1]解放军总医院医学实验动物中心,北京100853 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《实验动物科学》2015年第4期1-5,共5页Laboratory Animal Science

基　　金：国家科技支撑计划(No.2012BA139B04)

摘　　要：目的建立链脲佐菌素（streptozotocin,STZ）诱导巴马小型猪糖尿病模型。方法 10头小型猪随机分3组,设为对照组（2头）、实验1组（2头）和实验2组（6头）,分别静脉注射生理盐水、120 mg/kg的STZ和150 mg/kg的STZ。在应用STZ后24h内,每小时检测一次血糖,此后每天一次,1个月后处死动物,取胰腺做病理学检查。结果120 mg/kg的STZ可引起一过性糖尿病,血糖在17 d后逐渐恢复。150 mg/kg的STZ,引发3/6头小型猪严重的糖尿病,血糖维持在16.1~35.0 mmol/L之间,不能自发恢复;2/3头小型猪呈一过性高血糖,类似应用120 mg/kg的STZ组动物的变化;此外,还有1/6头动物未发生明显的高血糖。结论巴马小型猪一次性静注120 mg/kg的STZ可引起一过性糖尿病,150 mg/kg的STZ可引起严重的糖尿病,动物对STZ的反应个体差异很大。Objective To establish streptozotocin（ STZ）-induced type 1 diabetes mellitus model in Bama minipigs.Method 10 minipigs were divided into 3 groups,which were treated with normal saline（ 2）,120 mg / kg STZ（ 2）and 150 mg / kg STZ（ 6） respectively. The blood glucose was detected at 1 h interval during the first 24 h after treatment,and at one day interval during the following 30 days. The animals were sacrificed for pathological detection at the end of 30 day test. Result Transitorily high blood glucose was induced with 120 mg / kg STZ which the highest level reached 21. 3 mmol / L,and gradually decreased to normal after 17 days. Stable high blood glucose was induced with 150 mg / kg STZ which varied among 6. 1—35 mmol / L in 3 /6 animals,and 2 /3 animals induced transitorily high blood glucose like 120 mg / kg STZ treated animals,and 1 /6 animal showed normal blood glucose. Conclusion Mild diabetes mellitus was induced with 120 mg / kg STZ. Sever diabetes mellitus was induced with 150 mg / kg STZ with high variation among animals.

关 键 词：巴马小型猪 糖尿病 链脲佐菌素 

分 类 号：Q-331[生物学]