X射线对人周围血淋巴细胞Gadd45 mRNA表达影响  

X-ray irradiation affects Gadd45 mRNA expression in human peripheral blood lymphocytes

作　　者：王恰[1] 李明芳 杨爱初[1] 杨宇华[1] 梁晓阳[1] 严茂胜[1] 

机构地区：[1]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300

出　　处：《中国职业医学》2015年第6期640-644,共5页China Occupational Medicine

基　　金：广东省职业病防治重点实验室(2012A061400007);广东省职业病防治院课题(Z201004)

摘　　要：目的分析不同受照剂量和不同受照时间对人周围血淋巴细胞生长抑制和DNA损伤基因45（Gadd45）mRNA相对表达水平的影响,探讨Gadd45基因作为生物剂量计的可能性。方法 1剂量-效应关系研究。以0.00、0.10、0.25、0.50、1.00、2.00、3.00和5.00 Gy剂量X射线离体照射健康人周围血样,分离淋巴细胞进行检测;2时间-效应关系研究。以2.00 Gy剂量X射线离体照射健康人周围血样,分离淋巴细胞后分别于培养0、3、6、12、24和48 h时收获细胞。采用实时荧光定量聚合酶链反应法检测Gadd45 mRNA相对表达水平。结果 1 0.00-0.50 Gy剂量范围内,Gadd45 mRNA相对表达水平随照射剂量的增加而增加,回归方程为y=1.056 9＋7.132 2 x[决定系数（R^2）=0.992,P〈0.01]。0.50 Gy剂量组Gadd45 mRNA相对表达水平达到峰值,为0.00 Gy剂量组的4.53倍;0.50-5.00 Gy剂量范围内,Gadd45 mRNA相对表达水平呈波动性下降。22.00 Gy剂量照射后3-12 h,Gadd45 mRNA相对表达水平随照射时间的增加而增加,回归方程为y=-6.366 0＋4.965 0 x（R^2=0.932,P〈0.05）。照射后12 h时间组Gadd45 mRNA相对表达水平达到峰值,为0 h时间组的57.68倍;Gadd45 mRNA相对表达水平在照射后24-48 h大幅度下降,照射后48 h时间组为0 h时间组的2.08倍。结论 X射线照射可致Gadd45 mRNA表达上调,在一定剂量和时间范围内具有良好的剂量-效应和时间-效应关系;但Gadd45基因作为新型生物剂量计仍需进一步研究验证。Objective To analyze the relative expression level of Growth arrest and DNA damage gene（ Gadd） 45 mRNA of human peripheral blood lymphocytes after X-ray irradiation with different doses and time,and to explore the possibility of Gadd45 gene as a biological dosimeter. Methods i） Dose-effect experiment. The healthy human peripheral blood were irradiated by 0. 00,0. 10,0. 25,0. 50,1. 00,2. 00,3. 00 and 5. 00 Gy X-ray and the lymphocytes were separated for measurement. ii） Time-effect experiment. The healthy human peripheral blood were irradiated by 2. 00 Gy X-ray and the lymphocytes were separated and then cultivate for measurement after cultivation time of 0,3,6,12,24 and 48 hours. The real-time fluorescent quantitative polymerase chain reaction method was used to detect Gadd45 mRNA expression level.Results i） The relative expression level of Gadd45 mRNA increased with the increasing dose from 0. 00 to 0. 50 Gy. The linear regression equation was y = 1. 056 9 ＋ 7. 132 2 x（ determination coefficient was 0. 992,P〈0. 01）. The relative expression level of Gadd45 mRNA achieved the peak value when the irradiation dose was 0. 50 Gy,which was 4. 53 times of that with 0. 00 Gy dose irradiation. The relative expression level of Gadd45 mRNA gradually decreased in 0. 50-5. 00 Gy. ii） In the period of 3-12 hours after 2. 00 Gy of X-ray irradiation,the relative expression level of Gadd45 mRNA increased with the increasing exposure time. The linear regression equation was y =- 6. 366 0 ＋ 4. 965 0 x（ determination coefficient was 0. 932,P〈0. 05）. It achieved the peak value at the time of 12 hours,which was 57. 68 times of that with0-hour time group. During the time of 24-48 hours after irradiation,the relative expression level of Gadd45 mRNA decreased sharply,the 48-hour group was 2. 08 times of 0-hour time group. Conclusion X-ray irradiation up-regulated the expression level of Gadd45 mRNA. There was a good dose-effect and time-effect relation in specific dose and time range. Further study is needed to

关 键 词：X射线 生长抑制和DNA损伤基因45 生物剂量计 基因表达 电离辐射 

分 类 号：R144.1[医药卫生—公共卫生与预防医学] X591[环境科学与工程—环境工程]