葛根素、大豆素、槲皮素及芦丁对CCl_4诱导小鼠急性肝损伤保护作用的比较研究  

Comparative Study on Protection Effect of Puerarin, Soybean Element, Quercetin and Rutin of CCl_4-induced Acute Hepatic Injury Mice

作　　者：杨晓敏[1] 张天娇[1] 许可嘉 赵福建[1] 牛建昭[1] 李健[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《世界科学技术-中医药现代化》2015年第10期2024-2028,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：北京市支持中央高校共建项目-青年英才计划(YETP0790):基于改善钙平衡的芪参益气治疗心肌缺血血瘀证的机制研究;负责人:杨晓敏;北京中医药大学自主选题项目(2013-JYBZZ-JS-001):基于"AP-1/环加氧酶-2/前列腺素E2"信号通路探讨四乙酰化葛根素对大鼠类风湿性关节炎的抗炎机制;负责人:杨晓敏

摘　　要：目的:研究葛根素等4种类黄酮化合物的抗氧化活性,并探讨其构效关系。方法:用4种具有代表性分子结构的类黄酮化合物(大豆素、葛根素、槲皮素和芦丁)分别灌胃小鼠,7天后腹腔注射CCL4,建立小鼠急性肝损伤模型,禁食18 h后,取肝组织进行石蜡切片、苏木素-伊红(HE)染色,观察组织形态,采用全自动生化分析仪检测各组小鼠血清肝酶类成分丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、谷酰转肽酶(GGT)和碱性磷酸酶(ALP)的含量,并采用匀浆法检测肝组织中过氧化产物丙二醛(MDA)、抗氧化酶超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的含量。结果:肝组织病理学结果显示,与模型组相比,4种药物干预组小鼠肝组织损伤程度均减轻,但各干预组间未见显著性差异。血清生化分析结果显示,与模型组相比,葛根素干预组和槲皮素干预组小鼠血清中ALT、AST和GGT含量均明显下降(P<0.05或P<0.01),ALP含量无明显变化;大豆素干预组小鼠血清中仅ALT、AST含量明显下降(P<0.05或P<0.01);芦丁干预组小鼠血清中各酶活性均无明显变化。肝组织匀浆检测结果显示,与模型组相比,槲皮素干预组和芦丁干预组小鼠肝组织MDA含量明显下降(P<0.05),SOD和GSH-Px含量明显上升(P<0.05或P<0.01),而大豆素干预组和葛根素干预组仅可见GSH-Px含量明显上升(P<0.05或P<0.01)。结论:槲皮素的综合抗氧化能力优于大豆素、葛根素和芦丁,这可能与其结构特点有关。与其他3种化合物相比,槲皮素具有多羟基、羟基未成苷的结构特点,提示槲皮素的结构特点可能与其抗氧化活性密切相关。This study was aimed to compare the antioxidant activity of puerarin and 3 other flavonoid compounds, and to investigate their structure-activity relationship. The intragastric administration of 4 kinds of typical flavonoids compounds（soybean element, puerarin, quercetin and rutin） were given to mice, respectively. The model mice of acute hepatic injury were established with intraperitoneal injection of 0.1% carbon tetrachloride（CCl4） after 7 days. After 18 h fasting, liver tissues were removed. The histomorphology was observed after paraffin sectioning and hematoxylin-eosin staining. The activities of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, gamma-glutamyl transpeptidase（GGT） and alkaline phosphatase（ALP） in serum were detected with automatic biochemical analyzer. The content of malondialdehyde（MDA）, the activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in liver tissues were detected with homogenization. The pathological results of liver tissues showed that hepatic damages were decreased in all 4 medicine treatment groups compared with the model group, but there were no significant differences among these treatment groups. The results of blood serum bio-chemical analysis showed that compared with the model group, puerarin and quercetin could decrease the activities of ALT, AST and GGT in serum significantly（P〈0.05, or P〈0.01）. There were no content changes of ALP. In the soybean element group, only the activities of ALT and AST decreased obviously（P〈0.05, or P〈0.01）. There was no obvious change in the serum of mice in the rutin treatment group. The homogenate detection results of liver tissues showed that compared with the model group, quercetin and rutin significantly lowered MDA（P〈0.05）, increased SOD and GSH-Px（P〈0.05, or P〈0.01）; while soybean element and puerarin only improved GSH-Px levels（P〈0.05, or P〈0.01）. It was concluded that the antioxidant capacity of quercetin was better than th

关 键 词：葛根素 大豆素 槲皮素 芦丁 类黄酮化合物 抗氧化 构效关系 

分 类 号：R285.5[医药卫生—中药学]