滋补脾阴方药对脾阴虚痴呆大鼠脑内树突棘变化影响的研究  

Effects of Zi-Bu Pi-Yin Recipe on Dendritic Spines in Spleen-Yin Deficiency Dementia Model Rats

作　　者：隋华[1] 战丽彬[2,3] 嵇征鸿[4] 宫晓洋[5] 宫瑾[6] 王健[7] 

机构地区：[1]大连医科大学中西医结合基础研究所,大连116044 [2]大连医科大学附属二院,大连116023 [3]南京中医药大学基础医学院,南京210023 [4]大连市第二人民医院,大连116021 [5]大连医科大学附属一院,大连116021 [6]大连医科大学基础医学院,大连116044 [7]大连医科大学科技处,大连116044

出　　处：《世界科学技术-中医药现代化》2015年第10期2029-2033,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(30472255):滋补脾阴方药对兴奋性传递中树突棘调控的分子机制研究;负责人:战丽彬;高等学校博士学科点专项科研基金(优先发展领域)(20132105130001):蛋白质组学联合计算化学研究滋补脾阴方药改善糖尿病认知功能障碍的多成分多靶点调控机制;负责人:战丽彬;辽宁省高等学校创新团队资助项目(LT2014018):中医"脾主运化;主思藏意"理论的现代生物学研究;负责人:战丽彬

摘　　要：目的:通过对病证结合脾阴虚痴呆大鼠模型脑内不同区域树突棘变化的观察,探讨滋补脾阴方药(ZBPYR)对脑内树突棘变化的作用和影响。方法:采用经典方法建造脾阴虚模型,凝集态β-淀粉样蛋白1-40(β-Amyloid 1-40,Aβ1-40)定位注射到海马,建立脾阴虚痴呆模型,并应用ZBPYR干预。使用高尔基(Golgi)染色法,对动物模型脑内不同区域的树突棘进行染色,观察数量和形态。结果:脾阴虚痴呆组的海马各区和皮质的树突棘密度比脾阴虚组有所减少;与痴呆组和脾阴虚痴呆组比较,脾阴虚痴呆+ZBPYR组的大鼠海马各区和皮质的树突棘密度有所增加;与空白对照组比较,在痴呆组模型大鼠的脑内海马不同区域和皮质的树突棘密度下降。结论:脾阴虚痴呆大鼠不同脑区树突棘密度有不同程度减少,ZBPYR对学习记忆障碍的改善可能与维持不同脑区树突棘密度相关。This study was aimed to observe the changes of dendritic spine density in different regions of brain among spleen-yin deficiency dementia（SYDD） model rats, in order to investigate the effects of Zi-Bu Pi-Yin Recipe（ZBPYR） on dendritic spines. Spleen-yin deficiency（SYD） rats were modeled by classical method. And incubated β-Amyloid 1-40（Aβ1-40） was injected into the hippocampus of each rat to make SYDD model, which received the administration of ZBPYR. Golgi staining was used to stain dendritic spine in different regions of brain in rat model for the observation of the amount and shape. The results showed that dendritic spine density in different regions of hippocampus and cortex in SYDD group was reduced than that of the SYD group. Compared with the dementia group and the SYDD group, the dendritic spine density in different regions of hippocampus and cortex of the SYDD ＋ ZBPYR group was increased. Compared with the blank control group, the dendritic spine density in different regions of hippocampus and cortex in rats from the dementia group was reduced. It was concluded that there were different degrees of reducing in the dendritic spine density of different brain regions in SYDD group. ZBPYR improved the learning and memory impairment, which might be related to the maintenance of dendritic spine density in different brain regions.

关 键 词：脾阴虚痴呆 滋补脾阴方药 树突棘 Golgi染色 

分 类 号：R285.5[医药卫生—中药学]