补肾化痰方干预糖尿病认知障碍大鼠血清3-DG的研究  

Study on Intervention of Serum 3-deoxyglucosone by Bu-Shen Hua-Tan Fang in Rats with Diabetes Mellitus and Cognitive Impairment

作　　者：赵厚睿[1] 戴红[1] 杨旻[1] 

机构地区：[1]湖北中医药大学基础医学院,武汉430065

出　　处：《世界科学技术-中医药现代化》2015年第10期2039-2043,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：湖北省教育厅2013年高校科技能力创新工程项目(D20132004):补肾化痰法改善糖尿病大鼠认知障碍的机制研究;负责人:赵厚睿

摘　　要：目的:研究补肾化痰方对糖尿病大鼠学习记忆功能的干预情况。方法:将50只3月龄雄性Wistar大鼠随机分为对照组、模型组、补肾化痰方干预组(高、中、低剂量3组)。模型组和补肾化痰方干预组大鼠腹腔注射35 mg·kg-1剂量的链脲佐菌素,对照组大鼠腹腔注射相同体积的枸椽酸-枸椽酸钠缓冲液,补肾化痰方干预组大鼠分别使用20、10、5 g·kg-1剂量的补肾化痰方灌胃,30天后分别检测糖尿病大鼠的行为学和血清3-脱氧葡糖醛酮(3-DG)含量。结果:造模后模型组和补肾化痰方干预组大鼠血糖较对照组明显升高(P<0.01)。从第2天起,模型组大鼠到达站台所需时间较对照组所需时间明显延长,具有显著性差异(P<0.01)。从第4天起,补肾化痰方干预组各组大鼠所需时间均较模型组明显减少(P<0.05)。模型组大鼠血清3-DG含量较对照组明显增高(P<0.01)。补肾化痰方中剂量干预组大鼠血清中3-DG含量较模型组明显降低(P<0.05)。结论:补肾化痰方能够通过降低血糖及血清3-DG的水平,改善糖尿病大鼠的认知功能障碍。This paper was aimed to study the influence of Bu-Shen Hua-Tan Fang（BSHTF） on the learning and memory function of diabetes mellitus（DM） rats. A total of 50 3-month-old male Wistar rats were randomly divided into the control group, model group and 3 BSHTF groups（i.e., high-, middle-, and low-dose）. A total of 35 mg·kg^-1 streptozotocin（STZ） was intraperitoneally injected into the model and BSHT group. The same volume of citric acid-sodium citrate buffer solution was intraperitoneally injected into the control group. The intragastric administration of BSHTF at the dose of 20, 10, and 5 g·kg^-1 was given to the BSHTF groups, respectively. The behavior and content of serum 3-deoxyglucosone（3-DG） were detected among DM rats after 30 days. The results showed that the levels of serum glucose in the model group and the BSHTF groups were obviously higher than that of the control group（P〈0.01）. The swimming time to platform of rats in the model group was obviously prolonged compared to the control group with significant difference from the second day on（P〈0.01）. The required time in different BSHTF intervention groups was obviously decreased compared with the model group from the 4th day（P〈0.05）. The level of serum 3-DG in rats of the model group was obviously higher than that of the control group（P〈0.01）. The level of serum 3-DG in rats of the middle-dose BSHTF group was obviously decreased compared with the model group（P〈0.05）. It was concluded that BSHTF was able to improve the cognitive impairment among DM rats by reducing the levels of serum glucose and serum 3-DG.

关 键 词：补肾化痰方 糖尿病 认知功能障碍 3-脱氧葡糖醛酮 

分 类 号：R285.5[医药卫生—中药学]