基于NF-κB(p65)/CyclinE通路芪竹二术二草汤干预慢性萎缩性胃炎癌前期病变的研究  

Study on Therapeutic Effect and Mechanism of Qi-Zhu Er-Zhu Er-Cao Tang in Treatment of Chronic Atrophic Gastritis Precancerous Lesion Based on Pathway of NF-κB(p65) and CyclinE

作　　者：李梦莹[1] 徐陆周[1] 单兆伟[1] 

机构地区：[1]南京中医药大学第一临床医学院,南京210000

出　　处：《世界科学技术-中医药现代化》2015年第10期2119-2124,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：江苏省中医药管理局;2011-2012年度江苏省中医药科技项目计划(LZ110040):基于NF-κB(p65)/CyclinE通路益气养阴;清热活血方预防萎缩性胃炎癌前病变进展的研究;负责人:徐陆周

摘　　要：目的:观察芪竹二术二草方对萎缩性胃炎癌前病变的干预效果。方法:清洁级健康Wistar大鼠56只,随机分为阴性对照组(NC)、模型组、胃复春组(WFC)和仁术健胃颗粒组(RZ),每组10只,芪竹二术二草汤高剂量组(QZ-H)和低剂量(QZ-L)组,每组8只。模型组、WFC、RZ、QZ-H和QZ-L组采用化学诱癌物N-甲基-N’-硝基-N’-亚硝基胍(MNNG)诱发大鼠胃粘膜癌变,制备胃癌前期病变模型。WFC、RZ、QZ-H和QZ-L组大鼠分别按照0.39、3.2、54.4、13.6 g·kg-1剂量给予相应药物灌胃(ig),每天一次,连续28天;NC组和模型组大鼠给予等体积生理盐水。观察各组大鼠胃粘膜组织形态的改变,检测核转录因子(NF-κB,p65)和细胞周期蛋白E(CyclinE)的表达水平。结果:与阴性对照组相比,模型组大鼠炎细胞浸润、细胞异型增生程度及NF-κB/p65、CyclinE的表达明显增加,差异有统计学意义(P<0.01)。与模型组相比,WFC组大鼠NF-κB/p65及CyclinE表达减少,差异有统计学意义(P<0.05);RZ和QZ-L组大鼠NF-κB/p65及CyclinE表达明显减少,差异有统计学意义(P<0.01)。WFC、RZ、QZ-H及QZ-L组的复方总体有效率分别为50%、63.3%、43.3%及73.3%。结论:芪竹二术二草汤具有逆转萎缩性胃炎癌前期病变的作用,其作用机制可能是通过抑制原癌基因的转录和细胞周期调控蛋白的表达,从而防止肿瘤的发生发展。This study was aimed to observe the therapeutic effect and mechanism of Qi-Zhu Er-Zhu ErCao Tang（QZEZECT） in the treatment of chronic atrophic gastritis（CAG） precancerous lesion. A total of 56 clean grade healthy Wistar rats were randomly divided into 6 groups. In the negative control group（NC）, model group, Wei-Fu-Chun（WFC） group, Ren-Zhu Jian-Wei Ke-Li（RZJWKL, RZ） group, there were 10 rats in each group. In the high-dose QZEZECT（QZ-H） group and low-dose QZEZECT（QZ-L） group, there were 8 rats in each group. CAG/PLGC model was established by MNNG in the model group, WFC group, RZ group, QZ-H group and QZ-L group. Intragastric administration of corresponding decoctions at the dose of 0.39 g·kg^-1, 3.2 g·kg^-1, 54.4 g·kg^-1, 13.6 g·kg^-1 were given to rats in the WFC, RZ, QZ-H and QZ-L groups once a day for 28 consecutive days, respectively. The same volume of normal saline was given to the NC group and the model group. Histomorphological changes of gastric mucous membrane in rats of each group were observed. Expressions of NF-κB/p65 and CyclinE protein were detected. The results showed that compared with the NC group, the degrees of infiltration and dysplasia and expressions of NF-κB/p65 and CyclinE significantly increased in the model group with statistical significance（P〈0.01）. Compared with the model group, the expression of NF-κB/p65 and CyclinE in the WFC group reduced with statistical significance（P〈0.05）. The expression of NF-κB/p65 and CyclinE in the RZ and QZ-L group obviously reduced with statistical significance（P〈0.01）. The overall effective rates of WFC group, RZ group, QZ-H group and QZ-L group were 50%, 63.3%, 43.3% and 73.3%, respectively. It was concluded that QZEZECT can treat CAG precancerous lesion, which may take effect by improving and inhibiting pathologic changes of the transcription factor of inflammation.

关 键 词：NF-ΚB/P65 CYCLINE 芪竹二术二草汤 慢性萎缩性胃炎 胃癌前病变 

分 类 号：R273[医药卫生—中西医结合]