HPLC法测定3个产地酸枣仁中斯皮诺素和酸枣仁皂苷A、B的含量  

Determination of spinosin,jujubosides A and B in Ziziphi Spinosae Semen from three different origins by HPLC

作　　者：祝洪艳[1] 张力娜[1] 唐姗[1] 林海成 王国丽[1] 何忠梅[1] 郜玉钢[1] 张连学 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林省人参工程技术创新中心,长春130118

出　　处：《药物分析杂志》2015年第12期2099-2104,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：科技部科技支撑计划项目(2011BAI03B01;2011BAI03B010602);吉林省科技发展计划项目(20130102075JC);国家科技重大专项(2012ZX09304006);公益性行业科研专项(201303111);吉林省教育厅十二五项目(2014第60号)

摘　　要：目的：建立HPLC法测定吉林省常用的3个不同产地酸枣仁中斯皮诺素和酸枣仁皂苷A、B的含量,比较其含量差异,为对酸枣仁药材的选择提供依据。方法：采用Eclipse XDB-C18色谱柱（250 mm×4.6 mm,5μm）,柱温30℃。以乙腈-水为流动相梯度洗脱,检测波长为335 nm,检测斯皮诺素;以乙腈-水（34∶66）为流动相,检测波长为203 nm,同时检测酸枣仁皂苷A和B的含量。结果：斯皮诺素进样量在0.40-4.00μg（R2=0.999 6）范围内呈良好的线性关系,精密度、稳定性、重现性好,平均加样回收率为98.35%（RSD=1.52%）;酸枣仁皂苷A和酸枣仁皂苷B进样量分别在0.48-4.80μg（R2=0.999 3）和0.24-2.40μg（R2=0.999 9）范围内呈现良好的线性关系,平均加样回收率分别为98.99%（RSD=0.53%）和98.89%（RSD=0.69%）。3种酸枣仁中进口酸枣仁斯皮诺素含量最高,2种国产酸枣仁样品相差不大;然而,河北省产的酸枣仁皂苷A和B含量最高,其次是山西省,进口酸枣仁含量最低。结论：本文结果表明首次建立的梯度洗脱检测斯皮诺素含量方法准确、可靠,重复性好,可用于评价酸枣仁中斯皮诺素的含量。综合考虑,河北省产的酸枣仁可作为患者的首选药材。Objective： To establish an HPLC method for content determination of spinosin,jujubosides A and B in Ziziphi Spinosae Semen from three different origins that were mainly sold in each big pharmacy of Jilin province,and to provide scientific evidence for selection of Ziziphi Spinosae Semen. Methods： The assay of spinosin was performed on an Eclipse XDB-C18column（ 250 mm × 4. 6 mm,5 μm） by a new gradient elution program with acetonitrile-water. The column temperature was 30 ℃ and the detection wavelength of UV was 335 nm. Meanwhile,the HPLC-UV method was also used to detect contents of jujubosides A and B with mobile phase of acetonitrile-water（ 34∶ 66） and detection wavelength of 203 nm. Results： For spinosin,there was a good linear relationship in the range of 0. 40-4. 00 μg（ R2= 0. 999 6）,and the average recovery was 98. 35%,with RSD 1. 52%. In addition,its precision,stability and reproducibility were also good. The linear ranges of jujubosides A and B were 0. 48-4. 80 μg（ R2= 0. 999 3） and 0. 24-2. 40μg（ R2= 0. 999 9） respectively,and the average recovery was 98. 99% and98. 89%,with RSD of 0. 53% and 0. 69%. The highest content of spinosin was the Ziziphi Spinosae Semen in Burma,followed by Shanxi province and Hebei province,which were similar. The highest content of jujubosides A and B was the Semen in Hebei province,followed by Shanxi province,while the lowest was the Semen in Burma. Conclusion： The established method was accurate,reliable,and with good repeatability for detecting spinosin. Simultaneously considering the three typical components,Ziziphi Spinosae Semen in Hebei province could be the best choice for patients.

关 键 词：酸枣仁 酸枣仁皂苷A 酸枣仁皂苷B 斯皮诺素 产地比较 HPLC 

分 类 号：R917[医药卫生—药物分析学]