葛花解酲方对移植性肝癌小鼠外周血中免疫炎症因子含量的影响  

Effects of Gehuajiecheng Decoction on immune inflammation factor in peripheral blood of transplanted cancer of the liver mice

作　　者：李军[1] 唐东昕[2] 龙奉玺[1] 罗莉[2] 黄慧[2] 王镜辉[2] 郭斌[1] 杨柱[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550025 [2]贵阳中医学院第一附属医院,贵州贵阳550001

出　　处：《时珍国医国药》2015年第12期2845-2847,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81160417;No.81460697);贵州省研究生工作站计划项目(No.黔教研合JYSZ字[2014]018);贵州省科学技术厅-贵阳中医学院科学技术联合基金项目(No.黔科合LH字[2014]7336号)

摘　　要：目的通过研究葛花解酲方对移植性肝癌模型小鼠中IL-1、TNF-α水平变化的影响,探讨其可能的作用机制。方法将H22肝癌细胞株培养至对数生长期,接种到各造模组小鼠腋窝下,第2日开始给药,至造模周期结束后剥离瘤块,制作病理切片;取脾脏和胸腺,计算脾脏和胸腺指数;取血离心后用酶联免疫法检测血清中IL-1和TNF-α含量变化。结果模型组、葛花解酲方高、低剂量组IL-1、TNF-α分泌水平与空白组相比较均具有有统计学意义(P<0.05);模型组与葛花解酲方高、低剂量组相比较具有有统计学意义(P<0.05)。结论葛花解酲方在一定剂量下能够调节IL-1和TNF-α的分泌水平、改善BALB/c小鼠的免疫功能进而提示葛花解酲方的抑瘤作用可能与此相关。Objective Through research effect of Gehuajiecheng Decoction on IL-1 and TNF-α in peripheral blood of transplanted cancer of the liver mice,discuss the possible mechanisms. Methods The tumor-bearing were obtained by the axillary subcutaneous inoculation of H22 liver strains in right forelimp. After create model period,peel tumor lump and manufacture pathology section; Fetch Spleen and Thymus,calculation the Spleen index and thymus index; ELISA was used to examine the IL-1 and TNF-α content in peripheral blood after fetch blood centrifuge. Results The secretion level of model,high and low dose of Gehuajiecheng decoction groups compared with blank group have significant difference（ P〈0. 05）; High and low dose of Gehuajiecheng decoction groups compared with model group have significant difference（ P〈0. 05）. Conclusion Gehuajiecheng decoction can reduce the level of IL-1 and TNF-αin certain dose,improve the function of immune system,prompt the effect of restrain tumor with Gehuajiecheng Decoction.

关 键 词：葛花解酲方 肝癌 免疫炎症因子 IL-1 TNF-Α 

分 类 号：R285.5[医药卫生—中药学]