毛喉鞘蕊花的花粉形态及其活力测定研究  

Study on Pollen Morphology and Pollen Viability of Coleus forskohlii

作　　者：李捷[1] 刘君怡[1] 黄茜茜[1] 黄晓[1] 刘焱文[1,2] 余坤[1,2] 

机构地区：[1]湖北中医药大学药学院/湖北省中药资源与中药化学重点实验室,湖北武汉430065 [2]湖北福人药业股份有限公司博士后工作站,湖北通城437400

出　　处：《时珍国医国药》2015年第12期3005-3008,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31270405)

摘　　要：目的对毛喉鞘蕊花的花粉形态及其活力测定进行较系统研究,以期为该药材规范化种植培育优良种质提供实验依据。方法通过扫描电镜观察和分析,鉴定毛喉鞘蕊花的花粉形态特征;采用TTC染色法、I_2-KI染色法和醋酸洋红染色法及离体培养基法,对其花粉活力测定进行比较研究。结果其花粉粒为椭球形,具6条对称分布萌发沟,表面有网状纹饰,成熟花粉粒平均极轴长46.60μm,平均赤道轴长34.11μm;最佳花粉活力测定方法为离体培养基法,最佳离体生长条件为:150 g·L^(-1)PEG-4000+100 g·L^(-1)蔗糖+BK培养基,pH7.0,温度30℃,培养4~6 h。结论为毛喉鞘蕊花的药材鉴定和培育优良种质提供了实验数据。Objective To provide a reliable experimental data for the standardized cultivation of Coleus forskohlii by systematically studying pollen morphology and pollen viability. Methods Pollen morphology was observed by SEM; Detection of pollen viability was compared among TTC staining,I2-KI staining,Aceto-Carmine staining and in vitro gemination. Results The average polar and equatorial axis length of the mature pollen is 46. 60 μm,34. 11 μm; The best method to detect pollen viability is in vitro gemination,and the best cultivation system is 150 g·L^-1 PEG-4000 ＋ 100 g·L^-1 sucrose ＋ BK（ pH7. 0） for 4 to 6 hours at 30 ℃.Conclusion The data for identification and cultivation for Coleus forskohlii has been provided.

关 键 词：毛喉鞘蕊花 花粉形态 花粉活力 离体萌发 

分 类 号：R282.71[医药卫生—中药学]