Chronic caffeine exposure attenuates blast-induced memory deficit in mice  

作　　者：Ya-Lei Ning Nan Yang Xing Chen Zi-Ai Zhao Xiu-Zhu Zhang Xing-Yun Chen Ping Li Yan Zhao Yuan-Guo Zhou 

机构地区：[1]Molecular Biology Center, State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery and Daping Hospital,Third Military Medical University, Chongqing, China [2]Department Four, State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery and Daping Hospital,Third Military Medical University, Chongqing, China

出　　处：《Chinese Journal of Traumatology》2015年第4期204-211,共8页中华创伤杂志（英文版）

基　　金：We gratefully acknowledge Prof. Zhi-Yong Yin, senior engineers Xiao-Yan Li and Xin Ning for their technical support on the BST-I bio-shock tube. We thank Academician Zheng-Guo Wang for critically reading and editing the manuscript. This work was supported by the grants from National Natural Science Foundation of China （No. 81201461）, Key Project of Medicine and Health of PLA （No. 08G098） and the Natural Science Foundation of Chongqing, China （No.CSTC2012jjAI0107）.

摘　　要：Objective： To investigate the effects of three different ways of chronic caffeine administration on blast- induced memory dysfunction and to explore the underlying mechanisms. Methods： Adult male C57BL/6 mice were used and randomly divided into five groups： control： without blast exposure, con-water： administrated with water continuously before and after blast-induced trau- matic brain injury （bTBI）, con-caffeine： administrated with caffeine continuously for 1 month before and after bTBI, pre-caffeine： chronically administrated with caffeine for 1 month before bTBI and withdrawal after bTBI, post-caffeine： chronically administrated with caffeine after bTBI. After being subjected to moderate intensity of blast injury, mice were recorded for learning and memory performance using Morris water maze （MWM） paradigms at 1, 4, and 8 weeks post-blast injury. Neurological deficit scoring, glutamate concentration, proinflammatory cytokines production, and neuropathological changes at 24 h, 1, 4, and 8 weeks post-bTBl were examined to evaluate the brain injury in early and prolonged stages. Adenosine A1 receptor expression was detected using qPCR. Results： All of the three ways of chronic caffeine exposure ameliorated blast-induced memory deficit, which is correlated with the neuroprotective effects against excitotoxicity, inflammation, astrogliosis and neuronal loss at different stages of injury. Continuous caffeine treatment played positive roles in both early and prolonged stages of bTBI; pre-bTBI and post-bTBI treatment of caffeine tended to exert neu- roprotective effects at early and prolonged stages of bTBI respectively. Up-regulation of adenosine A1 receptor expression might contribute to the favorable effects of chronic caffeine consumption. Conclusion： Since caffeinated beverages are widely consumed in both civilian and military personnel and are convenient to get, the results may provide a promising prophylactic strategy for blast-induced neurotrauma and the consequent cognitive impairm

关 键 词：Brain injuries Caffeine Memory disorders Blast injuries 

分 类 号：U469.11[机械工程—车辆工程] X53[交通运输工程—载运工具运用工程]