中国一般人群外周血淋巴细胞微核率基准值分析：蒙特卡洛模拟法  

Reference value for micronucleus frequency of peripheral blood lymphocytes in general Chinese population： a method of Monte Carlo simulation

作　　者：滕晶晶[1,2] 段化伟[1] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所、中国疾病预防控制中心化学污染与健康安全重点实验室,北京100050 [2]安徽省疾病预防控制中心

出　　处：《中华劳动卫生职业病杂志》2015年第12期881-886,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金（81130050）;国家科技支撑计划（2014BAI12802）

摘　　要：目的估算中国一般人群外周血淋巴细胞微核率基准值，指导环境或职业化学接触人群的遗传毒性评价和风险分析。方法在中国知网（CNKI）数据库按照全文包含“微核”和“人”，PUBMED数据库中检索“cytokinesis—block micronucleus”和“CBMN”并选取“humans”和“adults”，检索2001至2014年公开发表的使用胞质分裂阻滞法（CBMN）进行微核检测并设立非遗传毒物接触人群对照的国内外研究文献，按照文献提供的微核率、标准差及样本量进行蒙特卡洛模拟，计算一般人群的微核率，并分析性别、吸烟和饮酒对微核率的影响。结果检索出符合条件的文献23篇，微核率均数最小为0．39‰，最大为25．3‰，对照组人数累计1623例（22～178例，平均70．6例），用蒙特卡洛模拟100次得出的微核率众数为0或1‰，P0为0o‰，如为1‰，氏为2‰～3‰，P75为5‰-6‰，氏为14‰～19‰；均数为4．36‰（4．22‰～4．57‰），采用95％单侧范围（x±1．64s）计算得出的参考值上限范围为13．46‰～14．75‰。结论中国一般人群外周血淋巴细胞微核率为4．36‰，四分位数范围为1‰-5‰或1‰～6‰，参考值上限为14．17％，且生活地区、性别、吸烟和饮酒习惯等方面均会对微核率产生影响。Objective To estimate the reference value for micronucleus frequency of peripheral blood lymphocytes in general Chinese population, and to guide the genotoxicity evaluation and risk analysis for populations exposed to environmental or occupational chemicals. Methods A full-text search was performed in CNKI with the key words of ＂micronucleus＂ and ＂human＂, and PubMed was searched with ＂cytokinesis-block mieronucleus＂, ＂CBMN＂, ＂humans＂, and ＂adults＂, to obtain the articles published at home and abroad from 2001 to 2014 in which eytokinesis-block micronueleus （CBMN） assay was applied for micronucleus detection and populations not exposed to genotoxins were established as a control. Monte Carlo simulation was performed based on the micronucleus frequency, standard deviation, and sample size provided in these articles to calculate the micronucleus frequency for general population and to analyze the influence of sex, smoking, and drinking on micronucleus frequency. Results A total of 23 articles were included in the final analysis. The minimum mean micronucleus frequency was 0.39‰, and the maximum mean micronucleus frequency was 25.3‰. There were 1623 subjects in the control group in total （range 22-178, mean 70.6）. Monte Carlo simulation was performed 100 times, and the mode of micronucleus frequency was 0 or 1‰; the values of P0, P25, P50, P75, and P95 were 0‰, 1‰, 2‰-3‰, 5‰-6‰, and 14‰-19‰, respectively; the mean value was 4.36‰ （range 4.22‰- 4.57‰）. With the application of one-sided 95% range （x±1.64s）, the upper limit of the range of reference value was calculated to be 13.46‰- ~14.75‰. Conclusion The micronucleus frequency of peripheral blood lymphocytes in general Chinese population is 4.36‰, the interquartile range is 1‰-5‰or 1‰-6‰, and the upper limit of reference value is 14.17‰. The factors of living area, sex, smoking, and drinking may influence micronucleus frequency.

关 键 词：微核 胞质分裂阻滞法 淋巴细胞 蒙特卡洛模拟 

分 类 号：R446.1[医药卫生—诊断学]