不同蒸制法对三七主根中皂苷的影响  

Effects of different steaming methods on saponins in taproots of Panax notoginseng

作　　者：武双[1,2] 崔秀明[1,2] 郭从亮 王承潇[1,2] 杨野[1,2] 曲媛[1,2] 杨晓艳[1,2] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]昆明市道地药材可持续发展利用重点实验室,云南昆明650500

出　　处：《中草药》2015年第22期3352-3356,共5页Chinese Traditional and Herbal Drugs

基　　金：云南省教育厅科学研究基金重点项目(2014Z029);云南省人才培养项目(KKSY201426015)

摘　　要：目的研究不同蒸制法对三七主根中皂苷的影响。方法采用比色法测定主根中总皂苷的量,采用HPLC法测定主根中单体皂苷的量。结果蒸制后主根中总皂苷及单体皂苷三七皂苷R1和人参皂苷Rg1、Re、Rb1、Rd的量均有不同程度的降低,而人参皂苷Rh1、Rh4、Rk3和20(S)-、20(R)-人参皂苷Rg3这5种单体皂苷的量均有不同程度的增加。结论不同蒸制法对三七中皂苷成分的影响不同,总皂苷和5种主要皂苷成分的量下降程度和新产生成分的量增加程度与蒸制时间和温度相关。该方法可用于三七炮制品中皂苷类成分的定量测定和质量控制,为三七"生打熟补"与物质变化之间的相关性研究提供依据,同时为研究稀有皂苷的积累规律提供了一定的理论基础。Objective To research the effect of different steaming methods on the saponins content in the taproots of Panax notoginseng. Methods Colorimetric method is used to determine the content of total saponins in the taproot of P. notoginseng, using high performance liquid chromatography（HPLC） method for determining the content of monomer saponins in the taproot of P.notoginseng. Results The contents of total saponins, notoginsenoside R1, and ginsenosides Rg1, Re, Rb1, and Rd in the taproot of P.notoginseng have been reduced to some extent, while the contents of five kinds of monomer saponins of ginseng, i.e. ginsenosides Rh1, Rh4, Rk3, 20（S）-Rg3, and 20（R）-Rg3 all have been increased in varying degrees after steamed. Conclusion The different steaming methods have the different influences to saponin composition in the taproot of P. notoginseng, the contents of total saponins and five main saponins could decrease and new generated monomer saponins could increase associated with the steaming time and temperature. This method can be used for the determination of the contents of saponin compounds and quality control in processed notoginseng products, and provide the basis for the study on the correlation between notoginseng ＂sheng da shu bu＂ and substance changes, while provide the certain theory basis for researching the accumulation rule of rare saponins.

关 键 词：蒸制法 比色法 总皂苷 HPLC法 单体皂苷 三七皂苷R1 人参皂苷Rg1 人参皂苷RE 人参皂苷Rb1 人参皂苷RD 人参皂苷RH1 人参皂苷Rk3 人参皂苷Rh4 20(S)-人参皂苷RG3 20(R)-人参皂苷Rg3 

分 类 号：R283.1[医药卫生—中药学]