f_2相似因子法评价银杏酮酯缓释微丸大类成分总黄酮和各类成分的体外释放相关性研究  

Correlation of in vitro release between total flavonoids and different ingredients in sustained-release pellets of Ginkgo biloba extract by f_2 fit factor method

作　　者：祝丽欣[1] 陈芝[1] 郭莹[2] 黄绳武[1] 石森林[1] 刘会[1] 熊阳[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江中医药大学生命科学学院,浙江杭州310053

出　　处：《中草药》2015年第23期3482-3488,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81473434;81274090);国家自然科学基金青年基金(81202926;81503206)

摘　　要：目的引入f_2相似因子法对银杏酮酯(GBE)缓释微丸的大类成分总黄酮和各类成分(包括黄酮类和萜内酯类)的释放曲线进行分析,以能较全面地评价其体外释放。方法运用紫外分光光度法、高效液相-质谱联用(HPLC-MS)测定GBE缓释微丸中大类成分(总黄酮)和各类成分(槲皮苷、异鼠李素、芦丁、槲皮素、银杏内酯A、银杏内酯B、银杏内酯C、白果内酯)的体外释放率,并进行f_2相似因子计算;采用扫描电镜法观察微丸释药前后的微观结构,结合方程拟合分别对微丸中总黄酮和各类成分的释药机制解析,以验证f_2相似因子法的可靠性。结果优化工艺的GBE微丸黄酮类和内酯类的各成分与大类成分总黄酮的f_2均大于50,说明大类成分总黄酮与各类成分体外释放曲线具有较好的相关性。释药机制解析进一步验证了该结果的可靠性。结论 f_2相似因子法可运用于多组分中药缓释制剂大类成分和各类成分的体外释放评价。Objective To find a new method to evaluate the in vitro release of Ginkgo biloba extract（GBE） sustained-release pellets, f_2 fit factor method was used to study the correlation of in vitro release between total flavonoids and different ingredients（including flavonoids and terpenoids）. Methods The release rates in vitro of total flavonoids and different ingredients（quercitrin,isorhamnetin, lutin, quercetin, ginkgolide A, ginkgolide B, ginkgolide C, and bilobalide） were detected by UV and HPLC-MS respectively, and then f_2 fit factor was calculated between total flavonoids and different ingredients. Also the micro-structures of pellets before and after drug release were detected by scanning electron microscopy（SEM）, which could explain the drug release mechanism combined with the fitted equation. Results All f_2 values were greater than 50 between the total flavonoids and different ingredients of the in vitro release from GBE pellets of optimized preparation, which indicated that there might be a good correlation between them. The drug release mechanism further verified the reliability of the results. Conclusion The f_2 fit factor method could be applied in the evaluation of in vitro release for multi-component sustained-release preparations of Chinese materica medicine.

关 键 词：f2相似因子法 银杏酮酯微丸 黄酮 萜类内酯 体外释放 槲皮苷 异鼠李素 芦丁 槲皮素 银杏内酯A 银杏内酯B 银杏内酯C 白果内酯 

分 类 号：R284.2[医药卫生—中药学]