基于ISSR的甘肃不同产区栽培当归遗传多样性研究  

Genetic diversity analysis on Angelica sinensis from different habitats in Gansu province based on ISSR

作　　者：朱田田[1,2,3] 晋玲[1,2] 张裴斯[1] 李应东[1] 杜弢[1,3] 何子清 

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃中医药大学中(藏)药资源研究所,甘肃兰州730000 [3]甘肃中医药大学药用植物遗传育种研究所,甘肃兰州730000 [4]甘肃岷归中药材科技有限公司,甘肃兰州730000

出　　处：《中草药》2015年第23期3549-3557,共9页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81360615);甘肃省科技计划自然科学基金(1308RJZA127);国家中医药行业科研专项课题(财社[2012]13号)

摘　　要：目的研究甘肃不同产区栽培当归的遗传多样性。方法应用ISSR分子标记技术对甘肃省41个居群的栽培当归样本进行分析,利用Popgene 32软件分析Nei’s基因多样性指数（H）等遗传信息参数,应用Ntsys软件构建亲缘关系UPGMA聚类图。结果 8条引物共检测到154个位点,其中多态性位点119个,多态位点百分率（PPB）为77.27%。栽培当归居群间的H为0.222 9,Shannon’s多样性信息指数（I）为0.337 4,种群间基因分化系数（Gst）为0.683 9,基因流（Nm）为0.2311,遗传距离变化范围0.042 9-0.327 8。结论甘肃栽培当归遗传多样性在物种水平上较高;居群间遗传多样性水平明显高于居群内;居群间遗传分化程度大,且基本无基因交流。Objective To investigate the genetic diversity of Angelica sinensis from different habitats in Gansu province. Methods ISSR markers were used to analyze the genetic diversity of 41 populations of A. sinensis. Nei＇s genetic diversity index（H） and other parameters of genetic information were calculated by Popgene 32. UPGMA dendrogram relationship was clustered by Ntsys. Results Eight primers produced 154 bands, among which 119 were polymorphic bands, the average percentage of polymorphie bands（PPB） was 77.27%. H and Shannon＇s information index（I） were 0.222 9 and 0.337 4, the genetic differentiation coefficient（Gst） and gene flow（Nm） were 0.6839 and 0.231 1 within the population levels. The genetic distance varied from 0.042 9 to 0.327 8. Conclusion The genetic diversity among species of A. sinensis is at higher level, but the level of genetic diversity between populations is higher than that within populations. There is a great degree of genetic differentiation among populations, and gene flow does not almost exist between populations.

关 键 词：甘肃 当归 栽培 ISSR 遗传多样性 

分 类 号：R282.12[医药卫生—中药学]