运动对大学生外周血T细胞Dtx1蛋白表达以及T细胞增殖与活性的影响  

Effect of Exercise on Expression of Dtx1 in T Cell,Proliferation and Activity of T Cells in College Students

作　　者：宋赫奕[1] 施春宇 张杨[1] 孙莉丽[1] 赵淑华[1] 

机构地区：[1]哈尔滨医科大学,黑龙江哈尔滨150081

出　　处：《北京体育大学学报》2015年第11期76-79,97,共5页Journal of Beijing Sport University

摘　　要：目的:探究体育运动是否影响大学生外周血T淋巴细胞Deltex 1(Dtx1)蛋白表达,以及对T细胞发育活性的影响。方法:将具有不同运动量及运动习惯的大学生志愿者分为3组(运动1组:>12 h/周,运动2组:>6 h/周,运动3组:<1 h/周),分别抽取外周血提取T淋巴细胞,MTT实验检测3组学生之间T淋巴细胞增殖能力差异,ELISA实验检测外周血IL-2与IL-10浓度差异,同时Western blotting实验检测3组学生之间T淋巴细胞内Dtx1蛋白表达差异。结果:3组成员T细胞增殖能力存在显著差异(P<0.05,运动1组>运动2组>运动3组);3组成员的外周血IL-2及IL-10浓度存在显著差异(P<0.05,运动1组>运动2组>运动3组);同时,3组成员的T淋巴细胞Dtx1蛋白表达存在显著差异(P<0.05,运动1组<运动2组<运动3组)。结论:体育运动可显著降低大学生T淋巴细胞内Dtx1蛋白的表达,显著增强T细胞的增殖发育能力以及活性。Objective： To explore the effect of exercise on expression of Deltex 1 （ Dtxl ） in T cell and developmentand activity of T cell in college students. Methods ： students volunteers were divided into three groups by exerciseamount and exercise habit （exercise group 1： more than 12 hours/week, exercise group 2： more than 6 hours/week, exercise group 3： less than lhours/week）. Peripheral blood were collected and T cells were extracted, theproliferation of T cells was detected by MTT assay, the expressions of IL-2 and IL-10 in peripheral blood were test-ed by ELISA, the expression of Dtxl in T cells was measured by Western Blotting. Results： There are significantdifferences of proliferation of T cells （P 〈 0.05, group 1 〉 group 2 〉 group 3 ）, levels of 1L-2 and IL-10 in pe-rlpheral blood （ P 〈 0. 05, group 1 〉 group 2 〉 group 3 ） , and expression of Dtxl in T cells （ P 〈 0.05, group 1〈 group 2 〈 group 3 ） among three groups.

关 键 词：体育运动 NOTCH信号通路 Dtx1 T淋巴细胞活性 增殖能力 大学生 

分 类 号：G804.7[文化科学—运动人体科学]