基于金纳米棒放大的高灵敏度纳米光纤生化传感器  

Highly Sensitive Optical Nanofiber Sensor Based on Gold Nanorod Amplification

作　　者：李明[1,2] 李凯伟 代方[1,2] 周文超[1] 舒风风[1,2] 吴一辉 

机构地区：[1]中国科学院长春光学精密机械与物理研究所,吉林长春130033 [2]中国科学院大学,北京100049

出　　处：《光学学报》2015年第12期60-65,共6页Acta Optica Sinica

基　　金：国家国际科技合作专项(2015DFG02620);国家863计划(2015AA042402);中国科学院科研装备研制项目(YZ201353);吉林省"双十工程"重大科技攻关项目(201402011GX)

摘　　要：为满足生物医学领域对传感器灵敏度和特异性的要求,利用金纳米棒对纳米光纤消逝场强的消光作用,提出基于金纳米棒标记放大的锥形光纤生化传感器。通过理论计算金纳米棒对光纤传输特性的影响,设计实验证明纳米光纤具有对单个金纳米棒的响应能力。采用双抗夹心法对PBS溶液中的羊IgG检测,检测下限可达0.02ng/mL,证实此种传感器具有极高的灵敏度和特异性。提出的纳米光纤生化传感器结构简单、响应迅速、成本低廉,在医学临床诊断、食品安全检测、环境监测领域具有重要的实用价值。In order to meet the requirements of high sensitivity and specificity of biomedical research, a new optical nanofiber biosensor using gold nanorods（GNR） as amplification labels is reported, based on extinction property of GNR. Optical influence of GNR attached to the surface of fiber is studied. Single GNR is successfully resolved in experiment. In this nanofiber biosensor, a sandwich assay strategy is utilized for detection. The limit of detection（LOD） of this sensor for Goat- Ig G is 0.02 ng/m L in PBS, which shows a great performance in sensitivity and specificity. The biosensor, reported in this work, has the advantages of simple detection scheme, fast response time,and ease of miniaturization, which might make this biosensor a promising platform for clinical cancer diagnosis,food safety detection and environmental monitoring.

关 键 词：传感器 纳米光纤 金纳米棒 单纳米粒子探测 生化检测 

分 类 号：O436[机械工程—光学工程]