PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix  

作　　者：Shan Yu Tiantian Su Huijun Wu Shiheng Liu Di Wang Tianhu Zhao Zengjun Jin Wenbin Du Mei-Jun Zhu Song Lin Chua Liang Yang Deyu Zhu Lichuan Gu Luyan Z Ma 

机构地区：[1]State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beo'ing 100101, China [2]State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China [3]University of the Chinese Academy of Sciences, Beijing 100049, China [4]School of Food Science, Washington State University, Pullman, WA 99164-6120, USA [5]Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technologi- cal University, Singapore 637551

出　　处：《Cell Research》2015年第12期1352-1367,共16页细胞研究（英文版）

摘　　要：Biofilms are surface-associated communities of microorganism embedded in extracellular matrix. Exopolysac- charide is a critical component in the extracellular matrix that maintains biofilm architecture and protects resident biofilm bacteria from antimicrobials and host immune attack. However, self-produced factors that target the matrix exopolysaccharides, are still poorly understood. Here, we show that PslG, a protein involved in the synthesis of a key biofilm matrix exopolysaccharide Psl in Pseudomonas aeruginosa, prevents biofilm formation and disassembles exist- ing biofilms within minutes at nanomolar concentrations when supplied exogenously. The crystal structure of PslG indicates the typical features of an endoglycosidase. PslG mainly disrupts the Psi matrix to disperse bacteria from biofilms. PslG treatment markedly enhances biofilm sensitivity to antibiotics and macrophage cells, resulting in im- proved biofilm clearance in a mouse implant infection model. Furthermore, PslG shows biofilm inhibition and disas- sembly activity against a wide range of Pseudomonas species, indicating its great potential in combating biofilm-related complications.

关 键 词：BIOFILM glycosyl hydrolase EXOPOLYSACCHARIDE PslG structure biofilm matrix PSEUDOMONAS 

分 类 号：U469.11[机械工程—车辆工程] X53[交通运输工程—载运工具运用工程]