兔实验性骨关节炎六种血清学生物标志物的变化  

Changes of six serum biomarkers of rabbits with experimental osteoarthritis

作　　者：石婷[1] 高戈[1] 阎祖炜[1] 魏丹[1] 高梓博[2] 高洁生[2] 周勇[3] 胡蓉[4] 

机构地区：[1]中南大学湘雅医学院医学检验系,湖南长沙410013 [2]中南大学湘雅二医院风湿免疫科,湖南长沙410011 [3]中南大学湘雅三医院骨科,湖南长沙410013 [4]中南大学湘雅医院妇产科,湖南长沙410008

出　　处：《细胞与分子免疫学杂志》2015年第12期1620-1623,1628,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：湖南省科技厅科技项目(2012FJ3122;2014SK3138);中央高校青年助推计划(20121200168);中南大学本科生自由探索计划(2282014bks194);2014年大学生创新创业计划(国家级)(201410533113)

摘　　要：目的定量检测新西兰大白兔血清中白细胞介素1β(IL-1β)、基质金属蛋白酶13(MMP-13)、白细胞介素6(IL-6)、2型胶原C端肽(CTX2)、环氧合酶2(COX2)和前列腺素E2(PGE2)随时间变化的关系(时效性),探讨联合6种血清学生物标志物对骨关节炎早期诊断的价值和意义。方法 40只新西兰大白兔,随机均分为实验组和对照组。实验组兔用关节腔注射胶原酶的方法制备实验性兔骨关节炎模型,对照组兔关节腔不注射胶原酶。用膝关节病理切片和X线检查及HE染色来检测造模是否成功。造模成功后每隔2周对两组兔血清中IL-1β、MMP-13、IL-6、CTX2、COX2和PGE2的水平进行检测。结果实验组各个时间点6种生物标志物的水平均高于对照组,尤其在第4周变化最为明显。结论联合检测血清中IL-1β、MMP-13、IL-6、CTX2、COX2、PGE2有助于骨关节炎的早期诊断。Objective To investigate the value and significance of six serum biomarkers to the early diagnosis of osteoarthritis by means of quantitative detection of interleukin-1 beta（ IL-1β）,matrix metalloproteinase 13（ MMP-13）,IL-6,C-terminal telopeptide of collagen type Ⅱ（ CTX2）,cyclooxygenase 2（ COX2） and prostaglandin E2（ PGE2） levels with time going on.Methods 40 New Zealand white rabbits were divided into experimental group（ n = 20） and control group（ n = 20）. Injection of collagenase into articular cavity was performed in the experimental group for modeling. No treatment was done in the control group. Pathological observation,X-ray examination and HE staining of knee joints were conducted to verify the establishment of modeling. IL-1,MMP-13,IL-6,CTX2,COX2 and PGE2 levels were detected every two weeks after modeling in the experimental group,and these biomarkers were also detected in the control group at the same time points. Results Levels of the six serum biomarkers in the experimental group were significantly higher than those in the control group at each time point after modeling and were the most distinguishing at the 4th week. Conclusion Combined detection of IL-1β,IL-6,CTX2,COX2 and PGE2 in serum is helpful for early diagnosis of osteoarthritis.

关 键 词：骨关节炎 白细胞介素1Β 基质金属蛋白酶13 白细胞介素6 Ⅱ型胶原C端肽 环氧化酶2 前列腺素E2 

分 类 号：R684.3[医药卫生—骨科学] R392.12[医药卫生—外科学]