神经调节蛋白1通过增强其受体磷酸化促进人冠状动脉平滑肌细胞的增殖和迁移  

Neuregulin-1 promotes proliferation and migration of human coronary artery smooth muscle cells by enhancing its receptor phosphorylation

作　　者：何飞连 桂春[1] 李浪[1] 陈力铨 齐彬[1] 

机构地区：[1]广西医科大学第一附属医院西院心内科,广西南宁530021

出　　处：《细胞与分子免疫学杂志》2015年第12期1643-1646,1650,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81160021);广西自然科学基金重点项目(2014GXNSFDA118024)

摘　　要：目的探讨神经调节蛋白1(NRG1)对人冠状动脉平滑肌细胞(HCASMC)增殖和迁移的影响。方法培养HCASMC,Western blot法检测HCASMC中表皮生长因子受体2(Erb B2)、Erb B3和Erb B4的表达以及Erb B2、Erb B3和Erb B4的磷酸化;MTT法检测不同浓度NRG1刺激对HCASMC增殖的影响,利用TranswellTM小室检测不同浓度NRG1刺激对HCASMC迁移能力的影响。结果 Erb B2、Erb B3和Erb B4均能在HCASMC中表达,加入NRG1处理后,与空白对照组比较,3个受体磷酸化水平均明显增加。不同浓度的NRG1干预对HCASMC增殖和迁移的影响不同,10 ng/m L的NRG1能明显促进HCASMC的增殖和迁移。结论 NRG1通过增强其受体Erb B2、Erb B3和Erb B4的磷酸化促进HCASMC的增殖和迁移,进而可能促进HCASMC在血管生成中的作用。Objective To explore the effect of neuregulin-1（ NRG1） on proliferation and migration of human coronary artery smooth muscle cells（ HCASMCs）. Methods HCASMCs were cultured in vitro. The expressions of human epidermal growth factor receptor 2（ Erb B2）,Erb B3 and Erb B4 and the phosphorylation of these receptors in HCASMCs were detected by Western blotting. The proliferation of HCASMCs treated with different concentrations of NRG1 was examined by MTT assay,and the migration ability of HCASMCs treated with different concentrations of NRG1 was tested by TranswellTMassay.Results Erb B2,Erb B3 and Erb B4 were expressed in HCASMCs. Compared with the control group,the phosphorylation levels of three receptors increased significantly in NRG1 treatment groups. The proliferation and migration abilities of HCASMCs treated with different concentrations of NRG1 were different,and 10 ng / m L NRG1 markedly promoted the proliferation and migration of HCASMCs. Conclusion NRG1 can promote the proliferation and migration of HCASMCs by enhancing the phosphorylation of Erb B2,Erb B3 and Erb B4,suggesting NRG1 may have promoting effect on the angiogenesis of HCASMCs.

关 键 词：神经调节蛋白1(NRG1) 人冠状动脉平滑肌细胞(HCASMC) 增殖 迁移 

分 类 号：R392-33[医药卫生—免疫学] Q253[医药卫生—基础医学]