贵阳地区夏秋季腹泻病例中致泻性大肠杆菌监测分析  

Surveilliance and analysis on Diarrheagenic Escherichia coli for infectious diarrhea cases in summer and fall in Guiyang districts

作　　者：韦小瑜[1] 游旅[1] 田克诚[1] 唐光鹏[1] 王定明[1] 

机构地区：[1]贵州省疾病预防控制中心,贵州550004

出　　处：《医学动物防制》2016年第1期35-37,41,共4页Journal of Medical Pest Control

基　　金：国家科技重大专项资金项目(2012ZX10004-212);贵州省卫生厅科学技术基金项目(gzwkj2013-1-079)

摘　　要：目的了解贵阳地区夏秋季感染性腹泻病例中致泻性大肠杆菌的分布状况,为该地区致泻性大肠杆菌的预防控制提供依据。方法收集2010年贵阳地区6家哨点医院的381例感染性腹泻病例的粪便标本,提取标本DNA,采用实时荧光PCR(RT-PCR)方法进行肠致病性大肠杆菌(Enteropathogenic E Coli,EPEC)、肠产毒性大肠杆菌(Enterotoxigenic E coli,ETEC)、肠粘附性大肠杆菌(Enteroadherent E coli,EAEC)、肠侵袭性大肠杆菌(Enteroinvasive E coli,EIEC)、肠出血性大肠杆菌(Enterohemorrhagic E coli,EHEC)的检测,并进行病原统计分析。结果 381例腹泻病例的粪便标本中致泻性大肠杆菌检出率为34.65%,其中检出率最高的是EAEC,为12.07%;其次是EPEC,检出率为9.45%,其中非典型EPEC占75.00%;ETEC检出率为9.19%,其中ETEC-ST占60.00%;EIEC检出率为3.94%;EHEC未检出;两种病原混合感染检出率为5.25%,以EPEC和EAEC混合感染为主,占40.00%。≤5岁组和>5岁组病例病原检出率分别为35.28%和30.91%,差异无统计学意义(P>0.05);≤5岁组病原EAEC检出率较高(P<0.05);>5岁组病原ETEC检出率较高(P<0.05)。男性病例检出率高于女性(P<0.05)。8月、9月检出率相对较高(P<0.001)。结论贵阳地区腹泻病例致泻性大肠杆菌感染以EAEC为主,其次是EPEC和ETEC,未发现有EHEC,8月、9月贵阳地区致泻性大肠杆菌的检出率较高。Objective To understand the constitution of Diarrheagenic Escherichia coli（ DEC） for infectious diarrhea cases in summer and fall in Guiyang districts,and to provide basis for the control and prevention of Diarrheagenic Escherichia coli. Methods Stool specimens of 381 infectious diarrhea cases were collected from 6hospitals. The RT-PCR was used to detect Diarrheagenic Escherichia coli,including Enteropathogenic E Coli（ EPEC）,Enterohemorrhagic E coli（ EHEC）,Enterotoxigenic E coli（ ETEC）,Enteroinvasive E coli（ EIEC）and Enteroadherent E coli（ EAEC）. The composement of Diarrheagenic Escherichia coli had been statistically analysed. Results The total rate of DEC detection was 34. 65%,in which the highest rate of detection was EAEC（ 12. 07%）,secondly EPEC（ 9. 45%）,atypical EPEC accounted for 75%; the rate of detection for ETEC was 9. 19%, in which ETEC-ST accounted for 60%; the rate of detection for EIEC was 3. 94%; EHEC was not detected. The rate of detection for mix infected Diarrheagenic Escherichia coli was5. 25%. The mix infected EPEC and EAEC was dominant,accounting for 40%. The rates of detection for ≤5 years old group and 5 years old group were respectively 35. 28% and 30. 91%（ P〈0. 05）. EAEC was dominant in ≤5 years old group（ P〈0. 05）,while ETEC in 5 years old group（ P〈0. 05）. August and September had the highest rate of detection of Diarrheagenic Escherichia coli（ P〈0. 001）. Conclusions The dominant Diarrheagenic Escherichia coli for infectious diarrhea cases was EAEC in summer and fall of 2010 in Guiyang districts,secondly EPEC and ETEC. EHEC was not detected EHEC. August and September had The highest rate of detection for DEC in Guiyang district.

关 键 词：感染性腹泻 肠致泻性大肠杆菌 荧光PCR 贵阳地区 

分 类 号：R378.21[医药卫生—病原生物学]